提供形式:in 3.2 M ammonium sulphate。
酶活定义: One Unit of α-D-Mannosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-D-mannopyranoside (5 mM) in MES buffer (100 mM) and CaCl2 (2.5 mM) at pH 6.5 at 40℃。
特异性: Hydrolysis of terminal non-reducing α-D-mannose in α-(1-3), α-(1-4) and α-(1-6)-D-mannosides。
最佳温度:40℃
最佳pH:6.5。