本公司提供科研Angiotensin II血管紧张素Ⅱ抗体，抗体质量可靠，订购Angiotensin II血管紧张素Ⅱ抗体请联系在线客服或者销售人员。
抗体参数如下>>>>
中文名称：血管紧张素Ⅱ抗体
英文名称：Anti-Angiotensin II
货号：bs-0587R
抗体来源：兔
克隆类型：多克隆
蛋白分子量：predicted molecular weight: 53kDa
纯化方法：affinity purified by Protein A
交叉反应：hu, mo, rat, hrs, shp
测试应用：ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
产品背景介绍：AGT, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.血管紧张素II是调节血压、血容量的重要因子，也是一种可能的神经介质。Ang II 控制血压和体液平衡，但现在进一步认识到Ang II还与炎性改变有关.产品图片Antigen: bs-0587P, 0.2ug/100ul Primary: Antiserum of Rabbit-Anti-Angiotensin II (bs-0587R) at the dilution of 500, 1000, 2000, 4000, 8000, 16000, 32000; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 5000; TMB(C-0024) staining; Read the data in MicroplateReader by 450nm. Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Angiotensin II Polyclonal Antibody, Unconjugated(bs-0587R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining