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肿瘤抑制基因-2

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MRCHolland
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2022-08-14 08:36
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北京孚博生物科技有限公司

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北京孚博生物科技有限公司
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产品属性
产品说明
位于阿姆斯特丹的 MRC Holland 由 Jan Schouten 博士于 1985 年创立，最初专注于生产限制酶和分子量标记。 1997 年，公司的重点转向开发 DNA 分析新技术。2002 年，发表了第一篇关于多重连接依赖性探针扩增 (MLPA®) 的论文。从那时起，MLPA 已成为拷贝数变异检测的金标准，市场上有超过 350 种探针混合物。
MRC Holland 继续创新，开发新的 MLPA 检测、改进现有检测以及最近引入的两项新技术。 2018 年，公司推出了首*个用于脊髓性肌萎缩 (SMA) 新生儿筛查的 Melt 检测。 2020 年，我们推出了 digitalMLPA™ 技术，该技术结合了 MLPA 值得信赖的质量和新一代测序的强大功能，可在一次反应中检测更多目标序列。公司本着为世界提供用于诊断和研究目的且易于使用、高质量且价格合理的遗传工具为使命，继续开拓创新。

厂家官网：http://www.mrcholland.com/

我们优势进口荷兰MRC Holland的仪器等产品，价格优惠，货期3-4周，质量和售后有保障！

了解更多产品和定制服务信息请留言，或直接与我们销售人员联系。
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下面主要介绍一下MRC Holland公司的MLPA以及digitalMLPA：
①MLPA

MLPA在研究和诊断中的作用：
MLPA® (Multiplex Ligation-dependent Probe Amplification) is the go-to technique for studying gene copy number variations (CNVs) associated with disease. Laboratories worldwide rely on MLPA for the diagnosis and research of genetic disorders and tumours. The power of the technique lies in its versatility: MLPA can be used to detect copy number changes in anything from complete chromosomes to single exons. MLPA is also used to detect DNA methylation changes (MS-MLPA) and is sensitive enough to discriminate aberrations in disease-causing genes from highly similar pseudogenes.
选择MLPA的理由：
CNVs play a role in hundreds of genetic disorders, and MLPA assays exist for even the rarest of them. Laboratories around the world use MLPA to ensure that deletions and duplications are adequately recognised. For disorders where CNVs make up the majority of mutations, MLPA is used as a first-line test. The same applies to disorders in which genetic analysis is complicated by pseudogenes: here, MLPA is used for its exceptional sensitivity to discriminate closely related sequences. In oncogenomics, MLPA is used to study somatic CNVs to optimise tumour profiling.
MLPA的原理：
MLPA probes：MLPA probemixes have probes that target a specific genomic sequence. An MLPA probe consists of two parts: a left and a right probe oligonucleotide (LPO and RPO). The LPO and RPO contain PCR primer and DNA hybridisation sequences. An additional stuffer sequence in the RPO gives each probe a unique length.
Sample denaturation and probe hybridisation：In the first step, purified sample DNA is denatured. This is followed by overnight incubation with MLPA probe oligos. The LPO and RPO parts of each probe hybridise to immediately adjacent target DNA sequences.
Probe ligation：The second step is the ligation of probe oligos that hybridised to immediately adjacent target sequences. No mismatches around the ligation site are permitted, making the ligation reaction highly specific. The number of probe ligation products is a measure for the number of target sequences in the sample.
Probe amplification：In the third step, ligated probes are amplified in a multiplex PCR using a single universal primer pair. Only ligated probes are exponentially amplified, making the removal of unbound and non-ligated probes unnecessary.
Fragment separation：The fourth step is fragment separation by capillary electrophoresis. PCR products are loaded onto a capillary electrophoresis device and separated by length. Each fragment corresponds to a specific MLPA probe.
Data analysis：The final step is data analysis by Coffalyser.Net. Relative copy numbers are determined by comparing the relative peak heights of reference probes and target probes in the test samples with those in reference samples with a known normal copy number. Advanced quality checks help to recognize unreliable data.

②digitalMLPA

digitalMLPA简介：
SALSA® digitalMLPA™ is similar to conventional MLPA, the gold standard for CNV detection of multiple DNA sequences, but with the ability to examine many more targets. digitalMLPA takes the guesswork out of NGS CNV calls by combining the MLPA technology you trust for CNV detection with the large number of targets you have come to expect with NGS. With digitalMLPA, you can examine more targets than with conventional MLPA, with decreased costs and turnaround times.
选择digitalMLPA的理由：
digitalMLPA is a new powerful tool to meet your copy number identification needs. Based on the trusted MLPA technology, digitalMLPA aims to make your CNV screening more efficient. With digitalMLPA, you have the capability to obtain reliable CNV data for up to 1000 sequences in a single reaction. The first SALSA digitalMLPA panel, D001 Hereditary Cancer Panel 1, offers gene coverage that exceeds that of ten conventional MLPA assays, thereby streamlining oncogenetic screening.
digitalMLPA工作原理：
digitalMLPA probes
digitalMLPA probemixes have probes that target a specific genomic sequence. A digitalMLPA probe consists of two parts: a left and a right probe oligonucleotide (LPO and RPO). The LPO contains a P7 PCR primer and a DNA hybridisation sequence. The RPO contains a sequence for distinct digitalMLPA read identification and a DNA hybridisation sequence. A Barcode Oligo (BO) is added to every reaction. The BO contains a unique sequence for sample identification, along with Rd1 and P5 primer sequences.
Sample denaturation and probe hybridisation
In the first step, purified sample DNA is denatured. This is followed by overnight incubation with digitalMLPA probe oligos. The LPO and RPO parts of each probe hybridise to immediately adjacent target DNA sequences.
Probe ligation
The next step is the ligation of probe oligos that hybridised to immediately adjacent target sequences. No mismatches around the ligation site are permitted, making the ligation reaction highly specific. The number of probe ligation products is a measure for the number of target sequences in the sample. With digitalMLPA, it is possible to detect the number of ligation events.
Probe amplification
Ligated probes are amplified in a multiplex PCR using a single P7/P5 primer pair. Only ligated probes are exponentially amplified, making the removal of unbound and non-ligated probes unnecessary.
Amplicon sequencing
PCR products are mixed in equal amounts, diluted, denatured, and loaded onto an Illumina sequencer. For each probe in all reactions, at least 400 single reads should be generated for reliable quantification.
Data analysis
The final step is data analysis by Coffalyser digitalMLPA. Coffalyser digitalMLPA generates two easy-to-interpret reports for each sample in your experiment: one with general details and one with sample results. These reports indicate if sample and reaction quality were sufficient and highlight detected aberrant regions.

部分产品列表如下：
货号中文描述英文描述规格
A054SALSA MLPA A054 Campylobacter MBiT mix 1 probemix 25/50/100R
A055SALSA MLPA A055 Campylobacter MBiT mix 2 probemix25/50/100R
ME001肿瘤抑制基因-1SALSA MLPA ME001 Tumor sup.-1 probemix 25/50/100R
ME002肿瘤抑制基因-2SALSA MLPA ME002 Tumor sup.-2 probemix 25/50/100R
ME004肿瘤抑制基因-4SALSA MLPA ME004 Tumor sup.-4 probemix 25/50/100R
ME011错配修复基因SALSA MLPA ME011 MMR probemix 25/50/100R
ME024肿瘤，皮肤黑色素瘤SALSA MLPA ME024 9p21 probemix 25/50/100R
ME028普瑞德威利症候群（PWS），安琪儿/天使人综合症（AS）SALSA MLPA ME028 PWS/AS probemix 25/50/100R
ME029脆性X染色体SALSA MLPA ME029 FMR1/AFF2 probemix25/50/100R
ME030Beckwith-wiedemann综合征（BWS），侏儒综合征（RSS）SALSA MLPA ME030 BWS/RSS probemix25/50/100R
ME031假性甲状旁腺机能减退（P*HP）、澳氏遗传性骨营养不良症（AHO）SALSA MLPA ME031 GNAS probemix 25/50/100R
ME032SALSA MLPA ME032 UPD7-UPD14 probemix 25/50/100R
ME033SALSA MLPA ME033 TNDM probemix25/50/100R
ME042SALSA MLPA ME042 CIMP probemix25/50/100R
ME043SALSA MLPA ME043 Lynch Syndrome probemix 25/50/100R
P002遗传性乳腺癌SALSA MLPA P002 BRCA1 probemix25/50/100R
P003非息肉性结肠癌SALSA MLPA P003 MLH1/MSH2 probemix 25/50/100R
P004SALSA MLPA P004 ERBB2 probemix 25/50/100R
P008Lynch综合征SALSA MLPA P008 PMS2 probemix25/50/100R
P010线粒体维护SALSA MLPA P010 POLG probemix 25/50/100R
P011血管性血友病/Von Willebrand病（VWD）1SALSA MLPA P011 VWF mix 1 probemix25/50/100R
P012血管性血友病/Von Willebrand病（VWD）2SALSA MLPA P012 VWF mix 2 probemix25/50/100R
P013α地中海贫血/智力低下SALSA MLPA P013 ATRX probemix 25/50/100R
P014肿瘤研究SALSA MLPA P014 Chromosome 8 probemix 25/50/100R
P015RETT综合征SALSA MLPA P015 MECP2 probemix 25/50/100R
P016林岛综合征，视网膜和中枢神经血管母细胞瘤病，VHL综合征SALSA MLPA P016 VHL probemix 25/50/100R
P017多发性内分泌肿瘤SALSA MLPA P017 MEN1 probemix 25/50/100R
P018特发性发育迟缓SALSA MLPA P018 SHOX probemix25/50/100R
P021脊髓性肌肉萎缩症（SMA）SALSA MLPA P021 SMA probemix 25/50/100R
P022Pelizaeus-Merzbacher病（PMD）、佩梅病SALSA MLPA P022 PLP1 probemix25/50/100R
P025海绵状脑白质营养不良症SALSA MLPA P025 Canavan probemix 25/50/100R
P026索托斯综合征(脑性巨大发育综合征)SALSA MLPA P026 Sotos probemix 25/50/100R
P027葡萄膜黑色素瘤SALSA MLPA P027 Uveal probemix 25/50/100R
P028淋巴组织细胞增生症(家族性)SALSA MLPA P028 FHL probemix 25/50/100R
P029SALSA MLPA P029 WBS probemix25/50/100R
P031范科尼氏贫血症（FA）1SALSA MLPA P031 FANCA mix 1 probemix25/50/100R
P032范科尼氏贫血症（FA）2SALSA MLPA P032 FANCA mix 2 probemix25/50/100R
P033腓骨肌萎缩症SALSA MLPA P033 CMT1 probemix 25/50/100R
P034杜氏肌营养不良症（DMD1）SALSA MLPA P034 DMD-1 probemix 25/50/100R
P035杜氏肌营养不良症（DMD2）SALSA MLPA P035 DMD-2 probemix 25/50/100R
P036广泛的端粒筛查-3SALSA MLPA P036 Subtelomeres Mix 1 probemix 25/50/100R
P037慢性淋巴细胞白血病（CLL）1SALSA MLPA P037 CLL-1 probemix25/50/100R
P038慢性淋巴细胞白血病（CLL）2SALSA MLPA P038 CLL-2 probemix 25/50/100R
P040慢性淋巴细胞白血病（CLL）SALSA MLPA P040 CLL probemix 25/50/100R
P041共济失调毛细血管扩张症（AT）1SALSA MLPA P041 ATM-1 probemix25/50/100R
P042共济失调毛细血管扩张症（AT）2SALSA MLPA P042 ATM-2 probemix 25/50/100R
P043息肉性结肠癌SALSA MLPA P043 APC probemix25/50/100R
P044神经纤维瘤病（NF2）SALSA MLPA P044 NF2 probemix 25/50/100R
P045遗传性乳腺癌SALSA MLPA P045 BRCA2/CHEK2 probemix25/50/100R
P046结节性硬化症SALSA MLPA P046 TSC2 probemix 25/50/100R
P047成视网膜细胞瘤（RB）SALSA MLPA P047 RB1 probemix25/50/100R
P048肢带型肌营养不良，肌原纤维肌病SALSA MLPA P048 LMNA/MYOT probemix 25/50/100R
P049肌酸转运蛋白，X-连锁SALSA MLPA P049 SLC6A8 - ABCD1 probemix 25/50/100R
P050先天性肾上腺增生症SALSA MLPA P050 CAH probemix 25/50/100R
P051帕金森病-1SALSA MLPA P051 Parkinson mix 1 probemix 25/50/100R
P052帕金森病-2SALSA MLPA P052 Parkinson mix 2 probemix 25/50/100R
P054眼科遗传性异常SALSA MLPA P054 FOXL2 TWIST1 probemix 25/50/100R
P055SALSA MLPA P055 PAH probemix25/50/100R
P056李佛美尼综合症（LFS）SALSA MLPA P056 TP53 probemix 25/50/100R
P057SALSA MLPA P057 FANCD2-PALB2 probemix 25/50/100R
P058远端脊肌萎缩症1型（DSMA1），SMARD1，dHMN6SALSA MLPA P058 IGHMBP2 probemix 25/50/100R
P059肌张力障碍SALSA MLPA P059 Dystonia probemix 25/50/100R
P060脊髓性肌肉萎缩症（SMA）SALSA MLPA P060 SMA probemix25/50/100R
P061无脑回畸形SALSA MLPA P061 Lissencephaly probemix 25/50/100R
P062SALSA MLPA P062 LDLR probemix25/50/100R
P063SALSA MLPA P063 FHIT-WWOX probemix 25/50/100R
P064智力低下， 3P-缺失，Williams, Smith- Magenis, Miller-Dieker, DiGeorge, Prader-Willi, Alagille, Saethre-Chotzen, SotosSALSA MLPA P064 MR-1 probemix25/50/100R
P065马凡氏综合征-1SALSA MLPA P065 Marfan-1 probemix 25/50/100R
P066马凡氏综合征-2SALSA MLPA P066 Marfan-2 probemix 25/50/100R
P067高林综合征（面耳脊柱病）SALSA MLPA P067 PTCH probemix 25/50/100R
P068线粒体3-羟基-3-甲基辅酶A合酶（mHMGS）SALSA MLPA P068 HMGCS2 probemix 25/50/100R
P069广泛的端粒筛查-4SALSA MLPA P069 Subtelomeres Mix 2A probemix 25/50/100R
P070广泛的端粒筛查-5SALSA MLPA P070 Subtelomeres Mix 2B probemix 25/50/100R
P071脑白质营养不良SALSA MLPA P071 LMNB1 PLP1 probemix25/50/100R
P072非息肉性结肠癌SALSA MLPA P072 MSH6 probemix25/50/100R
P074雄激素不敏感综合征（AIS）SALSA MLPA P074 AR probemix 25/50/100R
P075SALSA MLPA P075 TCF4 FOXG1 probemix25/50/100R
P076原发性肉碱/VLCAD SALSA MLPA P076 ACADVL-SLC22A5 probemix25/50/100R
P077遗传性乳腺癌SALSA MLPA P077 BRCA2 probemix 25/50/100R
P078乳腺癌SALSA MLPA P078 Breast tumor probemix25/50/100R
P079鸟氨酸氨基甲酰转移酶（OTC）SALSA MLPA P079 OTC probemix 25/50/100R
P080颅面疾病SALSA MLPA P080 Craniofacial probemix 25/50/100R
P081神经纤维瘤病-1SALSA MLPA P081 NF1 mix 1 probemix 25/50/100R
P082神经纤维瘤病-2SALSA MLPA P082 NF1 mix 2 probemix25/50/100R
P083上皮-钙粘连素/CDH1基因SALSA MLPA P083 CDH1 probemix 25/50/100R
P087遗传性乳腺癌SALSA MLPA P087 BRCA1 probemix 25/50/100R
P088少突神经胶质瘤SALSA MLPA P088 Glioma-1 probemix 25/50/100R
P089线粒体DNA耗竭综合征SALSA MLPA P089 TK2 probemix25/50/100R
P090遗传性乳腺癌SALSA MLPA P090 BRCA2 probemix 25/50/100R
P091SALSA MLPA P091 CFTR probemix 25/50/100R
P092弹力纤维性假黄瘤SALSA MLPA P092 ABCC6 probemix 25/50/100R
P093毛细血管扩张症（HHT），原发性肺动脉高压（PPH1）SALSA MLPA P093 HHT/PPH1 probemix25/50/100R
P094地中海热（MEFV）SALSA MLPA P094 MEFV probemix 25/50/100R
P095唐氏综合症，爱德华综合症，帕韬氏综合症（13,18,21,X,Y）SALSA MLPA P095 Aneuploidy probemix 25/50/100R
P096智力低下SALSA MLPA P096 MR-2 probemix 25/50/100R
P098SALSA MLPA P098 Wilson probemix25/50/100R
P099肌张力障碍、濑川氏病、肌阵挛 -肌张力障碍综合征SALSA MLPA P099 GCH1 TH-SGCE probemix 25/50/100R
P100肥厚型心肌病(家族性)SALSA MLPA P100 MYBPC3 probemix 25/50/100R
P100肥厚型心肌病(家族性)SALSA MLPA P100 MYBPC3 probemix 25/50/100R
P101着色斑性息肉消化道综合征（PJS）SALSA MLPA P101 STK11 probemix 25/50/100R
P102β-2地中海贫血SALSA MLPA P102 HBB probemix 25/50/100R
P103二氢嘧啶脱氢酶（DPYD）不足SALSA MLPA P103 DPYD probemix 25/50/100R
P104缅克斯病SALSA MLPA P104 Menkes probemix 25/50/100R
P105恶性脑胶质瘤SALSA MLPA P105 Glioma-2 probemix 25/50/100R
P106SALSA MLPA P106 MRX probemix 25/50/100R
P107多发性内分泌肿瘤SALSA MLPA P107 Neurometabolic probemix25/50/100R
P108Brugada综合症 /长QTSALSA MLPA P108 SCN5A probemix 25/50/100R
P109药物转运泵SALSA MLPA P109 ABCB4 probemix 25/50/100R
P110FCGR基因1q23.3SALSA MLPA P110 FCGR mix 1 probemix25/50/100R
P111FCGR基因1q23.3SALSA MLPA P111 FCGR mix 2 probemix 25/50/100R
P112PROS1不足SALSA MLPA P112 PROS1 probemix25/50/100R
P113范科尼氏贫血症互补（B组）SALSA MLPA P113 FANCB probemix 25/50/100R
P114先天性长QT综合征（LQT）SALSA MLPA P114 Long-QT probemix 25/50/100R
P116肢带型肌营养不良（LGMD）SALSA MLPA P116 SGC probemix25/50/100R
P117高胰岛素低血糖症（HHF1）SALSA MLPA P117 ABCC8 probemix25/50/100R
P118SALSA MLPA P118 WT1 probemix 25/50/100R
P120伴随脑铁积累的神经退化（NBIA）SALSA MLPA P120 PANK2/PLA2G6 probemix25/50/100R
P122神经纤维瘤病（NF1区域）SALSA MLPA P122 NF1 area probemix 25/50/100R
P124结节性硬化症SALSA MLPA P124 TSC1 probemix 25/50/100R
P125线粒体DNA（mtDNA）SALSA MLPA P125 Mitochondria probemix25/50/100R
P128细胞色素P-450SALSA MLPA P128 CYP450 probemix 25/50/100R
P129腓骨肌萎缩症SALSA MLPA P129 GJB1 probemix 25/50/100R
P130脑海绵状血管瘤（CCM）ASALSA MLPA P130 CCM mix-A probemix25/50/100R
P131脑海绵状血管瘤（CCM）BSALSA MLPA P131 CCM mix-B probemix 25/50/100R
P132Kallmann综合征，X-连锁SALSA MLPA P132 Kallmann-1 probemix 25/50/100R
P133Kallmann综合征，X-连锁SALSA MLPA P133 Kallmann-2 probemix25/50/100R
P136Gitelman综合征SALSA MLPA P136 Gitelman probemix25/50/100R
P137癫痫SALSA MLPA P137 SCN1A probemix 25/50/100R
P138葡萄糖转运酶1缺乏综合症SALSA MLPA P138 SLC2A1 probemix 25/50/100R
P139防御素SALSA MLPA P139 Defensin probemix25/50/100R
P140α地中海贫血SALSA MLPA P140 HBA probemix25/50/100R
P141侏儒症（CDLS）-1SALSA MLPA P141 NIPBL-1 probemix 25/50/100R
P142侏儒症（CDLS）-2SALSA MLPA P142 NIPBL-2 probemix 25/50/100R
P143腓骨肌萎缩症SALSA MLPA P143 MFN2 MPZ probemix25/50/100R

孚博生物常备库存产品
品牌、货号、名称、包装如下：
Toxin technology#BT202，SEB，1mg；
Toxin technology#BT202red，SEB(Staphylococcal Enterotoxins B)，1mg；
Toxin technology#ET404，SEE，100ug；
Toxin technology#DT303，SED，100ug；
Toxin technology#TT606，TSST-1，100ug；
Toxin technology#AT101，SEA，100ug；
Toxin technology#HT101，Alpha Hemolysin highly purified，100ug；
Toxin technology#HP101，Alpha Hemolysin - partially purified，10000Units；
IRA#SE-121-0.72mg，17β-ESTRADIOL，25片；
Listbio#150，Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae，1mg；
visual protein#IF01-4N，ImmunoFas Adjuvant，1套；
visual protein#HB01-1L，杂交瘤促进生长因子，50mg；
visual protein#BF20-50P，无蛋白封闭缓冲液，20片；
Research Diets#D12492，60%高脂饲料（research diets），12.5KG




Hooke Laboratories: 


SIGMA FBS胎牛血清常备库存：
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