内毒素:
Less than 0.1 EU per μg by the LAL method.

纯度:
>90% as determined by SDS-PAGE.

活性:
(1) Measured by its binding ability in a functional ELISA. Immobilized ActiveMax® Human IL-4 (Cat# IL4-H4218) at 5 μg/mL (100 μL/well) can bind Human IL-4 R alpha, His Tag (Cat# ILR-H5221) with a linear range of 0.01-0.1 μg/mL.(2) The bio-activity was determined by dose-dependent stimulation of the proliferation of TF-1 cells. The ED50 < 0.2 ng/mL, corresponding to a specific activity of >5000000 Unit/mg.

产品背景:
Interleukin-4, is a cytokine that induces differentiation of naive helper T cells (Th0 cells to Th2 cells). In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. Recently, researcher found that the cytokine IL-4 plays a key role in development of innate CD8+ T cells in the thymus of several gene-deficient mouse strains, including Itk, KLF2, CBP and Id3, without previous exposure to antigen.

产品描述:
ActiveMax® Human IL-4 contains no "tag", and has a calculated MW of 14.9 kDa. The predicted N-terminus is His 25. The reducing (R) protein migrates as 15 and 19 kDa in SDS-PAGE due to glycosylation.