凝血因子Ⅷ(F8)重组蛋白

¥1
上海钰博
进口/国产
2022-08-29 17:30

上海钰博生物科技有限公司

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上海钰博生物科技有限公司
顾磊
18321282235 021-60514606
shybio@126.com
产品属性
产品说明
凝血因子Ⅷ(F8)重组蛋白
适用生物 Canis familiaris; Canine (Dog,犬)
凝血因子Ⅷ(F8)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 18.2kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Coagulation Factor VIII (F8)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism speciesCanis familiaris; Canine (Dog)
Product No.RPB878Ca01
SourceProkaryotic expression
HostE.coli
Purity> 95%
UOM50ug
Predicted Molecular Mass18.2kDa
Predicted isoelectric point10.1
ApplicationsSDS-PAGE; WB; ELISA; IP.
Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
Subcellular LocationSecreted, extracellular space
ResiduesMet2191~Cys2337 (Accession # O18806) with N-terminal His-Tag
FormulationSupplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
凝血因子Ⅷ(F8)重组蛋白The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-MESKAISDAQ ITASSYLSSM LATWSPSQARLHLQGRTNAW RPQANNPKEW LQVDFRKTMK VTGITTQGVK SLLISMYVKE FLISSSQDGHNWTLFLQNGK VKVFQGNRDS STPVRNRLEP PLVARYVRLH PQSWAHHIAL RLEVLGC
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
凝血因子Ⅷ(F8)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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