5'-A^C T A G T-3' 3'-T G A T C^A-5'Concentration: 10u/ulSource: Bacillus coagulans VS 29-022Unit Definition: One unit is defined as the amount of B0808-05 required to digest 1ug of control DNA fragments in 1 hour at 37°C in 50ul of assay buffer. The control DNA is pUC19 DNA with inserted BcuI recognition site - Psp1406I fragments.Storage Buffer: Supplied as a liquid in 10mM Tris-HCl (pH 7.5 at 25°C), 300mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.Supplied with: R1625: Restriction Enzyme Buffer A, 10X: Suplied as a liquid in 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. Incubate at 37°C.Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods–the Storage Buffer should be used.