LO2细胞系:人正常肝细胞株 包售后 货号:GD-C4119668
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外著名大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难,最关键几点:1)所有的东西使用最好的,如果你用的血清、培养皿、培养基、胰酶什么的都是进口的,真的很难相信你会把细胞养坏;2)动作要快,胰酶消化,换液什么,细胞暴露在空气中的时间越少越好。另外,要掌握好胰酶消化时间,所谓的细胞状态差,很多时候是传代的原因;3)有时间的话,需要细胞计数,传相应数目的细胞到培养皿中,可以大致清楚细胞的生长曲线,不会临时要处理,手足无措;4)要做什么心里要非常清楚,合理安排时间,传代细胞的实验穿插进行,可以节省很多时间,也不会耽误别人的实验;5)个人的实验器具自己收一套,不要和别人混用,最近换了什么新的东西稍微记一下,试剂一旦怀疑污染,马上丢;6)传代细胞不能进去太多人,避免相互干扰。每天对待细胞比孝敬爹妈还用心,那真是捧在手里怕碎了,含在嘴里怕化了,看在眼里怕丢了,培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作,多喷喷酒精,要是对灭菌的东西不放心,自己包自己送自己烘干;2)不要和别人共用试剂耗材,自己准备一套;3)有可能的话在拿到新细胞的时候做好支原体衣原体检测;4)水浴锅很脏,生化培养箱要是得手动加湿的话盘里的水也会很脏,勤换(灭菌水加进去);5)晚上离开的时候最好给传代细胞照紫外,超净台是用完就开;6)最好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Selenious acid
PolyraM
Boc-(R)-α-(4-trifluoroMethylbenzyl)-Pro-OH
N-Methyl 5-broMopyridine-3-sulfonaMide
2-FLUORO-6-(TRIFLUOROMETHYL)BENZOYL CHLORIDE
1L-EPI-2-INOSOSE
Mesobilirubin
Cyclobutyl-4-fluorophenyl ketone, 98%
(S)-(o-(N-Benzylprolyl)amino)(phenyl)-methyleneiminoacetate
2-Bromo-5-fluorophenylacetic acid
BENZAL CHLORIDE
7-AVCA
(S)-(+)-1-Methyl-3-pyrrolidinol
Salicylaldoxime
2-BroMo-1-[5-(2-pyridinyl)-2-thienyl]-1-ethanone
Thiazole-2-carboxylic acid
2,2'-Ethylenedianiline
Z-Abu-oh
1-[3-(4-Chlorophenyl)-5-Methylisoxazol-4-yl]ethan-1-one, 97%
CIS-1 - 4-DICHLORO-2-BUTENE
3,4-Difluoroaniline
Angiotensin
Silver perchlorate
N,N'-trimethyleneurea
3-Nitrobenzyl alcohol
BENZYLTRIMETHYLAMMONIUM DICHLOROIODATE
Lumazine
2-Chlorobenzyl chloride
3-Nitrobenzoyl chloride, 98%
2-Chloro-6-Methylnicotinoyl chloride, 97%
Tri-n-butylphosphine, 95%
CouMarin 7
AZOBENZENE-4,4'-DICARBOXYLIC ACID DIMETHYL ESTER
N,N-Diethyl 3-broMo-4-MethylbenzenesulfonaMide
1,2,3-Benzenetricarboxylic acid hydrate
1,3-BIS(2,6-DI-I-PROPYLPHENYL)IMIDAZOL-2-YLIDENE
2-Selenophenecarboxaldehyde (6CI,7CI,8CI,9CI)
N-Formyl-DL-2-aminobutyric Acid
2,5-Dimethyl-3-hexyne-2,5-diol, 98%
N-Carboxyethylrhodanine
Methoxycarbonylmethyl(triphenyl)phosphonium Bromide
2-Bromo-1-(3,4-difluorophenyl)ethan-1-one
N-Benzylpiperidine-4-carboxaldehyde
2,2,3,4,4,4-Hexafluorobutyl acrylate
1'-Acetonaphthone
TetrabroMophenolphthalein ethyl ester
(+)-(5aR,10bS)-5a,10b-Dihydro-2-(2,4,6-triMethylphenyl)-4H,6H-indeno[2,1-b][1,2,4]triazolo[4,3-d][1,4]oxaziniuM Chloride Monohydrate
6-BroMoiMidazo[1,2-a]pyridine-3-carbaldehyde
1-(4-Chlorophenyl)-1-cyclopentanecarbonyl chloride, 97%
Terephthalic Acid
Methyl cis-9-Hexadecenoate
4-Bromo-benzenesulfonamide
C.I. Acid Red 37
Acetic-d3 acid-d
Cyclooctanone
4-CHLORO-8-(TRIFLUOROMETHYL)QUINOLINE
Riboflavin-5-phosphate sodiuM
BIS(TRIFLUORO-2,4-PENTANEDIONATO)COBALT(II)
N-Ethyl-3-carbazolecarboxaldehyde
Erbium(III) fluoride 99.9%
2-Amino-4-methyl-5-nitropyridine
H-Leu-OBzl.TosOH
Cerium(IV) sulfate hydrate, 99%
(HEPTAFLUOROBUTYRO)PHENONE
Nandrolone
3-(Trifluoromethoxy)phenol
2-Chloroethylamine hydrochloride
5-BroMo-2-furoic acid
2,2-Bis(4-oxocyclohexyl)propane
5-Carboxylthiophene-2-boronic acid pinacol ester
Anthracene, 99%
3-Cyano-5-fluorophenylboronic acid, 97%
2,2,3,3-Tetrafluoropropyl Methacrylate [stabilized with 2,2'-Methylenebis(6-tert-butyl-4-Methylphenol)]
N,N'-DibenzylethylenediaMine Diacetate
2,5-dimethylpyrrole
(2,6-dichlorophenyl)methylzinc chloride
4-oxoazetidine-2-carboxylic acid
2-Bromo-6-ethoxynaphthalene
UK ELISA Kit
Bid ELISA Kit
Human PI ELISA Kit
Human LRP6 ELISA Kit
STRAIL-R4 ELISA Kit
LTB ELISA Kit
IgG ELISA Kit
M-AChR M3 ELISA Kit
Human Insulin,INS ELISA Kit
PⅠNP ELISA Kit
The culture medium of Streptococcus vaccine
Starch Ammonium Salt Medium
Ethyl 2-aMinothiophene-3-carboxylate, 98%
trans-3-Hexene, 98%
4-(Ethylsulfonyl)benzeneboronic acid, 98+%
2'-AMinoacetophenone, 98%
Angiotensin
3-METHYL-1-BUTANETHIOL
3,4-Dihydroxythiophene-2,5-dicarboxylic acid diethyl ester, disodium salt
n-Heneicosane
2-Mercaptothiazole
2-(2-BROMOPHENYL)-1,3-DIOXOLANE
Pregabalin
StrontiuM selenide, 99.5% (Metals basis)
1,1-Cyclohexanediacetic acid, 98%
MCPA
3-[bis(2-hydroxyethyl)aMino]-2-hydroxy
Melatonine
DIMETHYL N,N-DIISOPROPYLPHOSPHORAMIDITE
3-Chloro-5-isopropoxyphenylboronic acid, pinacol ester
2-Methylpyridine-3-carboxaldehyde
Potassium Monoethyl Malonate
4-Methyl-3-nitrobenzenesulfonyl chloride
1-Fluoro-3,5-dimethoxybenzene, 98%
N-Ethylcarbazole-3-carboxaldehyde