适用生物 Homo sapiens (Human，人)
血管内皮生长因子121(VEGF121)检测试剂盒检测范围 15.63-1000pg/mL 灵敏度 5.7pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernatesand other biological fluids
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
血管内皮生长因子121(VEGF121)检测试剂盒ELISA Kit for Vascular Endothelial Growth Factor 121 (VEGF121)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | SEB851Hu |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernatesand other biological fluids |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 15.63-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 5.7pg/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Vascular Endothelial Growth Factor 121 (VEGF121).
No significant cross-reactivity or interference between Vascular Endothelial Growth Factor 121 (VEGF121) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Vascular Endothelial Growth Factor 121 (VEGF121) and the recovery rates were calculated by comparing the measured value to the expected amount of Vascular Endothelial Growth Factor 121 (VEGF121) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 88-102 | 93 |
| EDTA plasma(n=5) | 78-88 | 84 |
| heparin plasma(n=5) | 91-105 | 101 |
Precision
血管内皮生长因子121(VEGF121)检测试剂盒Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Endothelial Growth Factor 121 (VEGF121) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Endothelial Growth Factor 121 (VEGF121) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vascular Endothelial Growth Factor 121 (VEGF121) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-92% | 85-101% | 90-99% | 92-105% |
| EDTA plasma(n=5) | 90-97% | 80-94% | 84-94% | 98-105% |
| heparin plasma(n=5) | 96-104% | 80-101% | 99-105% | 86-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
血管内皮生长因子121(VEGF121)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Factor 121 (VEGF121). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vascular Endothelial Growth Factor 121 (VEGF121). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vascular Endothelial Growth Factor 121 (VEGF121), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vascular Endothelial Growth Factor 121 (VEGF121) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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