BKS DB Mouse Dermal Microvascular Endothelial Cells

ABC-TC5480

BKS DB Mouse

1 vial

Dry Ice

Liquid Nitrogen

BKS db Mouse Dermal Microvascular Endothelial Cells are isolated from the dermal microvascular of Mice homozygous for the diabetes spontaneous mutation (Lepr/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoRIemic. BKS db Mouse Dermal Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in AcceGen’s Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×106 cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.
BKS db小鼠皮肤微血管内皮细胞是从糖尿病自发突变纯合子小鼠(Lepr/db)皮肤微血管中分离得到的，Lepr/db表现为病态肥胖、慢性高血糖、胰腺β细胞萎缩和低血凝。BKS db小鼠真皮微血管内皮细胞在涂有明胶溶液的T25组织培养瓶中培养2分钟，然后在AcceGen的完全生长培养基中培养3-7天。文化被扩展。在运输之前，第3代细胞从烧瓶中分离出来，并立即在小瓶中冷冻保存。每瓶至少含有0.5×106个细胞每毫升。我们用于分离初级内皮细胞的方法是基于现有方法和我们专有的方法的结合开发的。在应用了涂有二抗的磁珠后，这些细胞被PECAM-1抗体预包覆。

For research use only

Mouse Primary Cells