绿色弹性蛋白酶测定试剂盒 荧光法、Green Elastase Assay Kit *Fluorimetric*详细如下：
Green Elastase Assay Kit 使用用 5-FAM 荧光团和 QXL™ 520 猝灭剂标记的天然底物弹性蛋白。标记弹性蛋白的蛋白水解切割产生明亮的绿色荧光，可以在激发/发射 = 488 nm/520 nm 下连续监测。荧光强度的增加与弹性蛋白酶活性成正比。该试剂盒不需要任何分离步骤，可用于连续测量弹性蛋白酶活性的动力学。
弹性蛋白酶催化弹性蛋白的水解，弹性蛋白是弹性纤维的主要成分，与胶原蛋白一起决定结缔组织的机械性能。弹性蛋白酶具有许多其他天然底物，包括蛋白聚糖、胶原蛋白和纤连蛋白。
Note: Thaw all kit components to room temperature before starting the experiments.
1X Assay buffer: For one 96-well plate, add 5 mL of 2X assay buffer (Component C) to 5 mL of deionized water.
Elastase substrate solution: Dilute elastase substrate (Component A) 100-fold in assay buffer. Prepare fresh substrate solution for each experiment.
Note: Dilute substrate according to the amount needed. Save the rest of the 100x substrate solution for future experiments.
Elastase diluents: Dilute one vial of the elastase enzyme (Component B) 40-fold in 1X assay buffer (refer to Table 2). The amount of enzyme is enough for a full 96-well plate. If not using the entire plate, adjust the amount of enzyme to be diluted accordingly.
Note: Prepare elastase diluents immediately before use. Do not vortex the enzyme solution.Prolonged storage or vigorous agitation of the diluted enzyme will cause denaturation. Store the enzyme solution on ice.
Elastase inhibitor (MeOSuc-Ala-Ala-Pro-Val-CMK): Dilute 10 mM inhibitor solution (Component D) 10-fold in assay buffer to get a concentration of 1 mM. Add 10 ul of the diluted compound into each of the inhibitor control well.
