520 组蛋白去乙酰化酶 (HDAC) 活性检测试剂盒 荧光法、520 HDAC Activity Assay Kit *Fluorimetric*详细如下：
520 HDAC 活性检测试剂盒提供了一种方便的两步均相程序，用于使用荧光底物测量 HDAC 活性。在第一步中，乙酰化底物与含有 HDAC 的样品一起孵育。底物的去乙酰化使其敏感，因此在第二步中与 HDAC 显影剂混合会产生荧光团，然后可以在 Ex/Em= 490 nm/520 nm 处检测到。试剂盒中包含的底物具有细胞渗透性，该检测可用于直接在 96 孔板中的细胞培养中测量 HDAC 活性，而无需耗时的细胞提取步骤。该试剂盒还可用于使用提取物或纯化酶对 HDAC 抑制剂进行高通量筛选。检测限：0.78 ug/ml
Add test compounds and diluted nuclear extract or enzyme solution to the microplate wells. For one well of a 96-well plate, the suggested volume of extract or enzyme solution is 40 uL and 10 uL of test compound.
Establish the following control wells at the same time, as deemed necessary:
Positive control contains HeLa nuclear extract without test compound.
Inhibitor control contains HeLa nuclear extract and Trichostatin A.
Vehicle control contains HeLa nuclear extract and vehicle used in delivering test compound (e.g. DMSO, concentration not to exceed 1%).
Test compound control contains assay buffer (Component D) and test compound. Some test compounds may themselves be fluorescent or interfere with the Ex/Em wavelengths and thereby give false results.
Substrate control contains assay buffer (Component D).
