HeLa细胞裂解物,HeLa Lysate(产品名称请以英文名称为准)【英文名称】:HeLa Lysate【货号】:1201【规格】:0.1 mg【保存条件】:Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.【简介】:HeLa lysate was prepared by homogenization in modified RIPA buffer (150mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mMethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1%Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed bycentrifugation. Protein concentration was determined with Bio-Radprotein assay. The HeLa lysate was boiled for 5 min in 1 x SDS samplebuffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate,0.01% bromophenol blue) containing 5% β-mercaptoethanol.HeLa细胞裂解物,HeLa Lysate 公司竭诚为广大科研用户提供最全面，最优质，价格最具优势的产品，免费为您提供全程技术指导，解决您的后顾之忧。公司供应各种高品质科研试剂及各种规格包装定制，请联系我们.