Recombinant Human UCH-L1/Ubiquitin carboxyl-terminal hydrolase isozyme L1 (C188)

产品说明(Description)

Recombinant Human Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 is produced by our E.coli expression system and the target gene encoding Met1-Ala223 is expressed with a 6His tag at the C-terminus.

Accession #: P09936

Known as: Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1; UCH-L1; Neuron Cytoplasmic Protein 9.5; PGP 9.5; PGP9.5; Ubiquitin Thioesterase L1; UCHL1

制剂（Formulation）

Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 250mM NaCl, 1mM DTT, 10% Glycerol, pH 7.5.

质量控制（Quality Control） 

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin:  Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

运输（Shipping）

The product is shipped on dry ice/polar packs.

Upon receipt, store it immediately at the temperature listed below.

保存（Storage）

Store at ≤-70°C, stable for 6 months after receipt.

Store at ≤-70°C, stable for 3 months under sterile conditions after opening.

Please minimize freeze-thaw cycles.

背景（Background）

Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 (UCHL1) belongs to the Peptidase C12 family. UCHL1 is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. UCHL1 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. UCHL1 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity.

电泳(SDS-PAGE)

FOR RESEARCH USE ONLY