Introduction

Human Laminin Beta 2 (LAMb2) ELISA Kit

Human Laminin Beta 2 (LAMb2) ELISA Kit

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to this index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for this index and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve

Recovery

Matrices listed below were spiked with certain level of recombinant the index and the recovery humanes were calculated by comparing the measured value to the expected amount of the index in samples.

Human Laminin Beta 2 (LAMb2) ELISA Kit

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concenthumanion of the index and their serial dilutions. The results were demonsthumaned by the percentage of calculated concenthumanion to the expected.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37℃;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37℃;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
DL-MYC-Mu 小鼠V-Myc骨髓细胞瘤病毒癌基因同源物(MYC) ELISA Kit mouse V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit Mus musculus mouse 0.156-10ng/mL 0.056ng/mL 96T 3800 12 months
DL-SCG3-Hu 人分泌粒蛋白Ⅲ(SCG3) ELISA Kit human Secretogranin III (SCG3) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.054ng/mL 96T 3880 12 months
DL-LAIR1-Hu 人白细胞关联免疫球蛋白样受体1(LAIR1) ELISA Kit human Leukocyte Associated Immunoglobulin Like Receptor 1 (LAIR1) ELISA Kit Homo sapiens human 0.313-20ng/mL 0.109ng/mL 96T 3705 12 months
DL-ODC-Mu 小鼠鸟氨酸脱羧酶(ODC) ELISA Kit mouse Ornithine Decarboxylase (ODC) ELISA Kit Mus musculus mouse 0.156-10ng/mL 0.059ng/mL 96T 3800 12 months
DL-TGM3-Mu 小鼠表皮转谷氨酰胺酶(TGM3) ELISA Kit mouse Transglutaminase 3, Epidermal (TGM3) ELISA Kit Mus musculus mouse 0.625-40ng/mL 0.255ng/mL 96T 3800 12 months
DL-TGM1-Mu 小鼠角质转谷氨酰胺酶(TGM1) ELISA Kit mouse Transglutaminase 1, Keratinocyte (TGM1) ELISA Kit Mus musculus mouse 0.313-20ng/mL 0.122ng/mL 96T 3800 12 months
DL-IFNa-Sh 绵羊干扰素α(IFNα) ELISA Kit Sheep Interferon Alpha (IFNa) ELISA Kit Ovis aries; Ovine Sheep 15.625-1000pg/mL 7.3pg/mL 96T 4430 12 months