【Molecular Wt.】36 kDa

【Description】The catalytic subunit of protein phosphatase 2A (PP2A) is inactivated by in vitro phosphorylation of Tyr-307 by receptor and nonreceptor protein tyrosine kinases. The catalytic subunit of PP2A is phosphorylated by tyrosine-specific protein kinases and associates with a variety of regulatory subunits. Phosphorylation is enhanced in the presence of the phosphatase inhibitor okadaic acid, consistent with an autodephosphorylation reaction. Phosphorylation is catalyzed by p60v-src, p56lck, epidermal growth factor receptors, and insulin receptors. Transient deactivation of PP2A might enhance transmission of cellular signals through kinase cascades within cells. In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases.

【Species Reactivity】Human;Mouse;Rat;zebrafish

【Tested applications】WB;ICC;IHC;IP

【Antibody type】Recombinant Rabbit monoclonal Antibody

【Immunogen】recombinant protein

【Positive control】A431, NIH/3T3, 293T, PANC-1, Hela, L6, rat kidney tissue, human tonsil tissue, human pancreas tissue, mouse brain tissue, mouse stomach tissue, mouse heart tissue.

【Conjugate】Non-conjugated

【Alternative names】PP2A A antibody
PP2A alpha antibody
PP2A B antibody
PP2A beta antibody
PP2A-alpha antibody
PP2AA_HUMAN antibody
PP2Ac antibody
PP2CB antibody
PPP2CA antibody
PPP2CB antibody
Protein phosphatase 2 catalytic subunit alpha isoform antibody
Protein phosphatase 2 catalytic subunit beta isoform antibody
Replication protein C antibody
RP C antibody
RP-C antibody
Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody
Serine/threonine protein phosphatase 2A catalytic subunit beta isoform antibody
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody

【Form】Liquid

【Stroage】Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

【Stroage Buffer】1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

【Isotype】IgG

【Purity】Protein A purified.

【Subcellular location】Cytoplasm, Nucleus, Chromosome, Cytoskeleton.

【Background References】1. Nardi F et al. Enhanced insulin sensitivity associated with provision of mono and polyunsaturated fatty acids in skeletal muscle cells involves counter modulation of PP2A. PLoS One 9:e92255 (2014).
2. Wang X et al. Microcystin (-LR) induced testicular cell apoptosis via up-regulating apoptosis-related genes in vivo. Food Chem Toxicol 60:309-17 (2013).
3. Cao Z et al. X-linked inhibitor of apoptosis protein (XIAP) regulation of cyclin D1 protein expression and cancer cell anchorage-independent growth via its E3 ligase-mediated protein phosphatase 2A/c-Jun axis. J Biol Chem 288:20238-47 (2013). 4, Lai CY et al. Depletion of 4E-BP1 and regulation of autophagy lead to YXM110-induced anticancer effects. Carcinogenesis 34:2050-60 (2013).

【DatasheetURL】http://www.huabio.com/upload/ET1611-54.pdf

【Uniprot ID】P62714(Human) P67775(Human) P62715(Mouse) P63330(Mouse) P62716(Rat) P63331(Rat)

【Raised in】Rabbit

【Clone No.】SN70-08