| 保存条件 | -20 |
| 数量 | 5000 |
| 供应商 | 昊鑫生物 |
| 规格 | 50 次x 50μl反应体系 |
产品组成、储存、浓度：
储存：－20 ℃ 避光保存至少6个月，使用前充分融解混匀。短期使用可放在4 ℃，避免反复冻融。
制品说明：
本制品本制品是采用SYBR® Green I嵌合荧光法进行Real Time PCR的专用试剂。已经将热启动HotMaster Taq DNA聚合酶、dNTP、特殊稳定剂、优化的反应缓冲液、BSA和SYBR® Green I等试剂预混成一种适合Real Time PCR反应检测用2×Premix Type试剂，具有灵敏度高、特异性强、稳定性好等特点。使用时只需加入模板和引物和水，便可在宽广的定量区域内得到良好的标准曲线，对目的基因进行准确定量检测，重复性好，可信度高。本品采用全新的Hotmaster Taq DNA 聚合酶，该酶不同于一般Hot-start 酶之处在于，一般的Hot-start 酶只在第一步温度升高之前封闭酶的活性，而Hotmaster Taq DNA 聚合酶是利用抑制剂通过温度调节方式封闭Hotmaster Taq DNA聚合酶的底物结合位点，温度低于40℃时，形成非活性的酶-抑制剂复合物，当温度升高至引物特异性的退火温度时，结合平衡向模板-特异性引物复合物形成方向移动，因此最大限度的减少PCR扩增全程中的非特异性扩增产物产生，大大提高了荧光定量PCR反应的精确性。
使用该产品发表的部分文章：
1. Qingkailing Injection Alleviates Experimental Autoimmune Uveitis in Rats via Inhibiting Th1 and Th17 Effector Cells. Biol. Pharm. Bull, 2012, 35(11) : 1991–1996 2. Expression Analysis of MAwuAG in Different Organs and Developmental Stages of Magnolia wufengensis. Chinese Bulletin of Botany, 2013, 48 (2): 1–1 3. Effect and Molecular Mechanism of DHA on Improving Rats Learning and Memory Ability, Food Science, 2012-11-9 4. Zinc Oxide Nanoparticles Induce Rat Retinal Ganglion Cell Damage Through Bcl-2,Caspase-9 and Caspase-12 Pathways Journal of Nanoscience and Nanotechnology. Vol. 13, 3769–3777, 2013 5. Zinc oxide nanoparticles decrease the expression and activity ofplasma membrane calcium ATPase, disrupt the intracellular calciumhomeostasis in rat retinal ganglion cells. The International Journal of Biochemistry & Cell Biology 45 (2013) 1849–1859 6. Zinc oxide nanoparticles inhibit Ca2+-ATPase expression in human lens epithelial cells under UVB irradiation. Toxicology in Vitro 27 (2013) 2117–2126 7. The organic extract from the Securidaca inhibits duck hepatitis B virus replication in vivo and hepatoprotective effect. Chin J Clinicians(Electronic Edition),December 1,2013,Vol.7,No.23 8. Disrupted calcium homeostasis is involved in elevated zinc ion-induced photoreceptor cell death. Archives of Biochemistry and Biophysics 2014, 560: 44–51 9. Molecular cloning and expression analysis of a myosin light chain 1 (MLC-1) gene from Indian meal moth Plodia interpunctella (Lepidoptera: Pyralidae). Entomological Research 2015, 45: 305-313 10. miR-143 inhibits tumor progression by targeting FAM83F in esophageal squamous cell carcinomaTumor Biol. 2016, DOI 10.1007/s13277-015-4760-9 11. An adenine nucleotide translocase (ANT) gene from Apostichopus japonicus; molecular cloning and expression analysis in response to lipopolysaccharide (LPS) challenge and thermal stress. Fish & Shellfish Immunology 2016, 49: 16 - 23 12. The Y137H mutation of VvCYP51 gene confers the reduced sensitivity to tebuconazole in Villosiclava virens. Scientific Reports 2015, 5:17575 | DOI: 10.1038/srep17575 13. Effects of the ninein like protein centrosomal protein on breast cancer cell invasion and migration. MOLECULAR MEDICINE REPORTS 2015, 12: 1659-1664 14. Molecular characterization of a 14-3-3 zeta gene from Plodia interpunctella: A potential marker for phylogenetic inference. Biochemical Systematics and Ecology 2015, 60: 171-176 |
