LS-F24635 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human LCN2 / Lipocalin 2 / NGAL in samples of Cell Culture Supernatants, Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of LCN2 / Lipocalin 2 / NGAL as low as 10 picograms per milliliter.
For the detection and quantification of endogenous levels of natural and/or recombinant Human NGAL proteins. Manual
Intended Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates Format
96-Well Strip Plate Detection
Colorimetric - 450nm (TMB) Measurement
Quantitative Detection Range
156 - 10000 pg/ml Sensitivity
10 pg/ml Storage
Short term: 4°C; Long term: see manual. Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Kit Components
User Manual
96-well Microplate
Detection Reagents
Wash Buffer
Substrate
Stop Solution
Adhesive Plate Sealers
Background
Lipocalin-2 (LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase. The LCN2 gene is located at 9q34 and contains 7 exons. The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions. The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long. LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. Restrictions
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