The Nuclear Extraction kit can be used for 20 reactions extracting nuclear extract from tissue culture cells grown on 10cm plates or 10 reactions using tissue. The kit comes complete with the following reagents listed below

• 1X Buffer A
• 1X Buffer B
• 100 mM DTT
• Protease Inhibitor
• Phosphatase Inhibitor I
• Phosphatase Inhibitor II

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Our cancer cell isolation method only takes a couple of hours, and involves just a few rounds of incubation, washing, and centrifugation. Using the same amount of starting biopsy material required for conventional methods, this technique can be used to isolate a much greater quantity of cancer cells.

Purify any population of cancer cells

The ability to obtain uncontaminated cancer cell samples is one of the major bottlenecks in the study of tumor development and cancer biology. Tumor biopsies from cancer patients and animal tumor models often contain a heterogeneous population of cells that include normal tissue, blood, and cancer cells. This mixed population makes diagnosis and valid experimental conclusions difficult to obtain and interpret.

Typically, cancer cells are isolated from biopsy samples via microdissection, a time-consuming technique that requires costly equipment and surgical precision. Unlike these techniques, our Cancer Cell Isolation Kit requires no specialized skill set and no expensive equipment, and can be used to isolate any cancer cell type.