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Pierce Protein Extraction Kits, Thermo Scientific

Quickly and easily isolate membrane, nuclear, or cytoplasmic proteins using a microcentrifuge tube protocol. This protocol involves cell lysis in hypotonic buffer and eliminates freeze/thaw cycles. Cross-contamination between fractions is generally less than 10%. Resulting proteins are compatible with most downstream applications, including SDS-PAGE, Western blotting, and protein assays.