| 规格 | 150ul |
RNA interference is a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. The introduction of a specific small interfering RNA (siRNA) strand prevents the translation of a target protein message through RNA degradation. SignalSilence® Control siRNA (Unconjugated) is a siRNA sequence that will not lead to the specific degradation of any cellular message. It is intended to serve as a negative control for experiments using targeted siRNA transfection. This duplex contains the same scrambled sequence used in SignalSilence® Control siRNA (Fluorescein Conjugate) #6201.
RNA干扰技术是一种通过向细胞中输入双链RNA分子,选择性沉默基因表达的方法。一种特定的小干扰RNA的引入可以通过降解RNA来阻止目的蛋白的翻译。SignalSilence ® Control siRNA (未标记)是一种小干扰RNA(siRNA),不能导致细胞信号的特定降解,而用来作为靶siRNA转染的阴性对照。这种双链RNA与SignalSilence ® Control siRNA (Fluorescein Conjugate) #6201具有相同的密码序列。
Western blot analysis of extracts from HeLa cells 48 hours following transfection with 100 nM non-targeted SignalSilence® Control siRNA (Unconjugated). Protein expression levels are measured using Akt (pan) (C67E7) Rabbit mAb #4691 (left), β-Actin (13E5) Rabbit mAb #4970 (middle) or p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (right). Western blot分析用100 nM non-targeted SignalSilence ® Control siRNA (Unconjugated) 转染48小时的HeLa细胞提取物。分别用 Akt (pan) (C67E7) Rabbit mAb #4691 (左), β-Actin (13E5) Rabbit mAb #4970 (中) or p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (右)检测蛋白的表达水平。