pEGFP-N2 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm;emission maximum = 507 nm.) pEGFP-N2 encodes the GFPmut1 variant which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr

载体信息：
启动子：CMV
复制子：pUCori,f1ori
终止子：SV40poly(A)
载体别名：pEGFPN2, pEGFP N2
载体分类：哺乳细胞表达载体，荧光蛋白报告载体
载体大小：4737bp
载体标签：C-EGFP
原核抗性：卡那霉素(Kanamycin)
筛选标记：新霉素(Neomycin)
克隆菌株：DH5α
表达宿主：哺乳细胞
培养条件：37℃，LB培养基
5' 测序引物：CMV-F：5'-CGCAAATGGGCGGTAGGCGTG-3'
3' 测序引物：EGFP-N：5'-CGTCGCCGTCCAGCTCGACCAG-3'

储存条件：-20℃