储存条件：冷冻（-20℃）

使用说明

To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.

技术规格

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1 μg of Lanbda DNA(dcm-)in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sites	TGG↑CCA
来源	Micrococcus species 20
Assayed on	Lanbda DNA(dcm-)
Optimal buffer	W?(10mMTris-HCl(pH 8.5 at 25℃);10mMMgCl2;100 mM NaCl;1 mM DTT.)+?BSA
Optimal temperature	37℃
Storage conditions	10mMTris-HCl(pH 7.5);200 mM KCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol;Store at -20℃.
Ligations	After 2-fold overdigestion with enzyme 80%of the DNA fragments can be ligated and recut.More complete ligation can be reached by using 10%PEG
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a.of enzyme for 16hoursat 37℃.
Methlation effect	Cleaved of DNA is impaired by overlapping Dcm methylation(CmCWGG):TGGCCAGG.
Thermal inactivation	enzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible Ends	BalI,MlsI,MluNI,MscI