TWEAK,short for TNF-related weak inducer of apoptosis,is also known as TNFSF12 and DR3LG.It is a type II transmembrane protein belonging to the TNF superfamily.It is expressed widely in many tissues,such as the heart,skeletal muscle,spleen and peripheral blood.Binding of TWEAK to its receptor TWEAKR induces NF-κB activation,chemokine secretion and apoptosis in certain cell types.TWEAK has also been reported to promote endothelial cell proliferation and migration,thus serving as a regulator of angiogenesis.

基本信息：

Species	Human
Source	CHO
Measured Molecular Weight	20～22kDa,observed by reducing SDS-PAGE.
Purity	> 95% as analyzed by SDS-PAGE and HPLC.
Formulation	Lyophilized after extensive dialysis against PBS.
Reconstitution	Reconstituted in ddH2O or PBS at 100μg/ml.
Endotoxin Level	<0.2 EU/μg,determined by LAL method.

Sequence：
RKTRARRAIA AHYEVHPRPG QDGAQAGVDG TVSGWEEARI
NSSSPLRYNR QIGEFIVTRA GLYYLYCQVH FDEGKAVYLK
LDLLVDGVLA LRCLEEFSAT AASSLGPQLR LCQVSGLLAL
RPGSSLRIRT LPWAHLKAAP FLTYFGLFQV H

储存条件：
Lyophilized recombinant Human TWEAK remains stable up to 6 months at lower than -70℃ from date of receipt.Upon reconstitution,Human TWEAK should be stable up to 1 week at 4℃ or up to 2 months at -20℃.

生物活性：
ED50 <1ng/ml,measured in a cell cytotoxicity assay using HTB-38 (HT-29) cells.