分子量：Approximately 13.4kDa,a single non-glycosylated polypeptide chain containing 123 amino acids.
储存条件：冷冻（-20℃）

概述

Midkine,also named MK,MK1,NEGF 2,is belonging to the neurotrophic and developmentally-regulated heparin-binding molecules family.It is encoded by the MDK gene.The Midkine protein includes five intrachain disulfide bonds which hold two domains and there are three antiparallel beta-sheets in each domain.A chondroitin sulfate proteoglycan,protein-tyrosine phosphatase zeta(PTPzeta),is a receptor for MK.MK promotes the growth,survival,and migration of various cells,and plays roles in neurogenesis and epithelial mesenchymal interactions during organogenesis.The predicted molecular weight is approximately 13.3 kDa,based on a mature peptide length of 118 amino acid residues in the mouse and 121 amino acid residues in the human.Across species,MK shows 87%identity between the human and murine proteins.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格

外观	Sterile Filtered White lyophilized(freeze-dried)powder.
纯度	＞97%by SDS-PAGE and HPLC analyses.
来源	Escherichia coli.
生物活性	Fully biologically active when compared to standard.The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 0.1-10ng/ml.
成分	Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH7.4.
内毒素	Less than 1 EU/μg of rHuMidkine as determined by LAL method.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml.Stock solutions should be apportioned into working aliquots and stored at ≤ -20?C.Further dilutions should be made in appropriate buffered solutions.
氨基酸序列	VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD