分子量:Protein A is a cell wall component produced by several strains of Staphylococcus aureus that consists of a single polypeptide chain and contains little or no carbohydrate.Recombinant Protein A is produced in E.Coli and functions essentially the same as n
储存条件:冷冻(-20℃)
应用 The Protein A molecule contains four high-affinity(Ka =108/mole)binding sites capable of interacting with the Fc region from IgG of several species including human and rabbit.Optimal binding occurs a pH 8.2,although binding is also good at neutral or physiological conditions(pH 7.0-7.6).
使用说明 This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格 | 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | >97%by SDS-PAGE analyses. |
| 来源 | Escherichia coli. |
| 成分 | Lyophilized with no additive. |
| 内毒素 | Less than 0.1 EU/μg of Protein A,His as determined by LAL method. |
| 复溶 | Dissolve in distilled water or saline. |
| Specificity | The interaction between Protein A and IgG is not equivalent for all species.Even within a species,Protein A interacts with some subclasses of IgG and not others.For instance,human IgG1,IgG2 and IgG4 bind strongly,while IgG3 does not bind.There are |