分子量：Approximately 15.1kDa,a single non-glycosylated polypeptide chain containing 135 amino acids.
储存条件：冷冻（-20℃）

概述

Glial cell-derived neurotrophic factor is a founding member of the GDNF family of ligands(GFL)and has been shown to interact with GFRA2 and GDNF family receptor alpha 1.It is a small protein that potently promotes the survival and morphological differentiation of various neuronal.It may also modulate local neuronal effects in distal regions of the motor neuron.GDNF Recombinant rat GDNF(monomer)contains 135 amino acids residues,which is a disulfide-linked homodimer and it shares 99%and 93%a.a.sequence identity with mouse and human GDNF.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格

外观	Sterile Filtered White lyophilized(freeze-dried)powder.
纯度	＞97%by SDS-PAGE and HPLC analyses.
来源	Escherichia coli.
生物活性	Fully biologically active when compared to standard.Measured by its ability to support the survival and stimulate neurite outgrowth of dissociated chick embryonic dorsal root ganglia(DRG)neurons.The ED50 for this effect is typically 1 - 3ng/mL.
成分	Lyophilized from a 0.2μmfiltered concentrated solution in 20mMPB,500 mM NaCl,pH 6.4.
内毒素	Less than 1 EU/μg of rRtGDNF as determined by LAL method.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions.
氨基酸序列	MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCEAAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDSLV YHILRKHSAK RCGCI