| 货号 | Z15802 |
| 规格 | 50μl | 100μl | 150μl |
| 抗体名称 | Anti-DCC Antibody |
| 指标别名 | Colorectal cancer suppressor;CRC18;CRCR1;DCC;IGDCC1;Netrin receptor DCC;Tumor suppressor protein DCC |
| 克隆性 | Polyclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,IHC,ELISA |
| 基因名称 | DCC |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | E.coli-derived human DCC recombinant protein (Position:Q851-F1447). |
| 实际分子量 | 180KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | Deleted in Colorectal Carcinoma,also known as DCC,is a protein which in humans is encoded by the DCC gene.It is mapped to 18q21.2.This gene encodes a netrin 1 receptor.The transmembrane protein is a member of the immunoglobulin superfamily of cell adhesion molecules,and mediates axon guidance of neuronal growth cones towards sources of netrin 1 ligand.The cytoplasmic tail interacts with the tyrosine kinases Src and focal adhesion kinase (FAK,also known as PTK2) to mediate axon attraction.The protein partially localizes to lipid rafts,and induces apoptosis in the absence of ligand.The protein functions as a tumor suppressor,and is frequently mutated or downregulated in colorectal cancer and esophageal carcinoma. |
| Uniprot ID | P43146 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 (ELISA)： 1:100～1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
