| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof T7 DNA in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | A↑CATGT |
| 来源 | E.coli strain that carries the cloned Pci I gene from Planococcus citreus SE-F45 |
| Assayed on | T7 DNA |
| Optimal buffer | O?(50mMTris-HCl(pH 7.6 at 25℃);10 mM MgCl2;100 mM NaCl;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 10 mM Tris-HCl(pH 7.5);250mMNaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;50%glycerol;Store at -20℃. |
| Ligations | After 10-fold overdigestion with enzyme 90%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof T7 DNA with 20 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Not blocked by ACmATGT methylation.|Blocked by?mACATGT methylation. |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | BspLU11I,PscI |
