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| 货号 | Z20898 |
| 规格 | 50μl | 100μl | 150μl |
| 抗体名称 | Anti-MAPK1/3 Antibody |
| 指标别名 | ERK;ERK 2;ERK2;ERT1;MAP kinase 1;MAP kinase 2;MAP kinase isoform p42;MAPK 1;MAPK 2;MAPK1;MAPK2;p40;p41;p41mapk;p42 MAPK;P42MAPK;PRKM1;PRKM2 |
| 克隆性 | Polyclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,IHC |
| 基因名称 | MAPK1/MAPK3 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | A synthetic peptide corresponding to a sequence at the N-terminus of human MAPK1/3(171-184aa ARVADPDHDHTGFL),identical to the related rat and mouse sequences. |
| 实际分子量 | 41KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | MAPK1(ERK2) shares high homology with MAPK3(ERK1).MAP kinase phosphatase as a locus of flexibility in a mitogen-activated protein kinase signaling network.Mitogen-activated protein(MAP) kinases [also known as Erks] have been established to function as important mediators of signal transduction by growth factor receptors.ERK1/ERK2-dependent activation of endogenous ribosomal transcription,while inactivation of ERK1/ERK2 causes an equally immediate reversion to the basal transcription level.ERK1/ERK2 was found to phosphorylate the architectural transcription factor UBF at amino acids 117 and 201 within HMG boxes 1 and 2,preventing their interaction with DNA.Mutation of these sites inhibited transcription activation and abrogated the transcriptional response to ERK1/ERK2. |
| Uniprot ID | P28482/P27361 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
| 基因名全称 | mitogen-activated protein kinase 1 |
| 蛋白名全称 | Mitogen-activated protein kinase 1(MAP kinase 1/MAPK 1) |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
