| 抗体名称 | Anti-NFKB2 Antibody |
| 指标别名 | DNA binding factor KBF2;H2TF1;LYT 10;LYT10;NF kappa B;NFKB2;NFKB2;p100;Oncogene Lyt 10;p100 |
| 克隆性 | Polyclonal |
| 检验物种 | human |
| 应用范围 | WB |
| 基因名称 | NFKB2 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | A synthetic peptide corresponding to a sequence at the N-terminus of human NFkB p100(1-21aa MESCYNPGLDGIIEYDDFKLN). |
| 实际分子量 | 52KD/100KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | NFKB2(Nuclear Factor Kappa-B,Subunit 2),also known as NFKB or p52/p100 SUBUNIT,is a protein that in humans is encoded by the NFKB2 gene.Liptay et al.(1992) mapped the gene for what they called the p49/p100 subunit of NFKB(NFKB2) to chromosome 10 by Southern blot analysis of panels of human/Chinese hamster cell hybrids.By fluorescence in situ hybridization(FISH),they confirmed the localization and mapped the gene with greater resolution to 10q24.NFKB2 appears to be the same as LYT10.Claudio et al.(2002) showed that bone marrow(BM) cells from Nfkb2-deficient mice,but not Nfkb1-deficient mice,failed to increase relative and total IgD-positive transitional-1(T1) stage B cells in response to Baff.In vivo,however,Nfkb2-deficient mice did generate mature B cells,but at reduced numbers.Mice of the aly/aly strain,which are naturally deficient in Nik,and mice of the A/WySNJ strain,which have a mutation in Baffr,also failed to produce T1 B cells in response to Baff.Baff stimulation enhanced expression of Bcl2 in T1 B cells,thereby promoting B-cell survival,and caused the processing of the p100 form of Nfkb2 to p52,which again required Baffr and Nik,but not Nemo(IKKG).Immunoblot analysis showed that BM cells contained primarily p100. |
| Uniprot ID | Q00653 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
| 基因名全称 | nuclear factor of kappa light polypeptide gene enhancer in B-cells 2(p49/p100) |
| 蛋白名全称 | Nuclear factor NF-kappa-B p100 subunit |
| 推荐稀释比 |
Western blot(WB) 1:500～2000 |
