产品货号: jlcR6576 英文名称: RBBP6 中文名称:视网膜母细胞瘤结合蛋白6抗体 别名: Retinoblastoma binding protein 6; RBBP6; MY038; P2P R; P2PR; p53 associated cellular protein of testis; PACT;Proliferationpotentialrelated protein; Protein P2P R; Protein P2PR; Protein RBQ 1; Protein RBQ1; RB binding Q protein 1; RBBP 6; RBQ 1; RBQ1; Retinoblastoma binding protein 6 isoform 3; Retinoblastoma binding Q protein 1; SNAMA; RBBP6_HUMAN. 研究领域:肿瘤细胞生物细胞凋亡细胞周期蛋白肿瘤细胞生物标志物 抗体来源: Rabbit 克隆类型: Polyclonal 交叉反应: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, 产品应用: WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 分子量: 201kDa 细胞定位:细胞核细胞浆 性状: Lyophilized or Liquid 浓度: 1mg/ml 免疫原: KLH conjugated synthetic peptide derived from human RBBP6:1201-1350/1792 亚型: IgG 纯化方法: affinity purified by Protein A 储存液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存条件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. PubMed: PubMed 产品介绍background: The retinoblastoma tumor suppressor (pRB) protein binds with many other proteins. In various human cancers, pRB suppresses cellular proliferation and is inactivated. Cell cycle-dependent phosphorylation regulates the activity of pRB. Retinoblastoma binding protein 6 binds to underphosphorylated but not phosphorylated pRB. Multiple alternatively spliced transcript variants that encode different isoforms have been found for this gene.
Function: E3 ubiquitin-protein ligase which promotes ubiquitination of YBX1, leading to its degradation by the proteasome. May play a role as a scaffold protein to promote the assembly of the p53/TP53-MDM2 complex, resulting in increase of MDM2-mediated ubiquitination and degradation of p53/TP53; may function as negative regulator of p53/TP53, leading to both apoptosis and cell growth.
Subunit: Interacts with p53/TP53 and RB1 (By similarity). Interacts also with MDM2 and YBX1. Interacts with NEK6.
Subcellular Location: ucleus, nucleolus. Chromosome. Cytoplasm, cytoskeleton, centrosome. Note=Colocalizes with mitotic chromosomes. Co-localizes with NEK6 in the centrosome.
Tissue Specificity: Highly expressed in the placenta and testis. Expressed at lower levels in the brain, heart, kidney, liver and lung. Overexpressed in esophageal cancer.
Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated by NEK6.
