成功案例

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磷酸化是蛋白质翻译后修饰的最为重要的形式。磷酸化蛋白在细胞信号转导、细胞的分化、增值、周期调控、新陈代谢、转录和翻译、蛋白降解和细胞生存等方面发挥着重要的作用。我公司利用TiO2富集方法和nanoLC-MS/MS在线连用进行磷酸化蛋白质组学研究，建立了TiO2在线富集鉴定复杂样品的磷酸化分析体系。



磷酸化蛋白质组分析策略

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仪器

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nanoACQUITY UPLC超高效液相色谱系统
Synapt High Definition Mass Spectrometry 质谱仪 （Waters 公司）

参考文献

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1.Bernd Bodenmiller et al. PhosphoPep-a database of protein phosphorylation sites in model organisms. Nature Biotechnology .2008,1339-1340.
2.Alberto Paradela et al. Advances in the Analysis of Protein Phosphorylation. J. Proteome Res. 2008, 1809-1818.
3.Jason Ptacek et al. Global analysis of protein phosphorylation in yeast. Nature.2005, 679-684.
4.Sean A Beausoleil et al. A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Nature Biotechnology. 2006, 1285-1292.
5.Philip Cohen et al. The origins of protein phosphorylation.Nature Cell Biology .2002, E127-E130
6.Jeffrey A et al. Mechanisms of specificity in protein phosphorylation. Nature Reviews Molecular Cell Biology. 2007, 530-541.
7.Johnson LN. The regulation of protein phosphorylation. Biochem Soc Trans. 2009, 627-641.