荧光素酶检测试剂

QUANTI-Luc™

报告基因检测试剂

QUANTI-Luc™ is provided as packs of individually sealed pouches.
• rep-qlc1: 2 pouches of QUANTI-Luc™
• rep-qlc2: 5 pouches of QUANTI-Luc™
Each pouch contains everything needed to prepare 25 ml of reagent allowing the preparation of 500 wells of a 96-well plate.

QUANTI-Luc™ Procedure

1. Prepare QUANTI-Luc™ reagent by adding 25 ml water to the contents of one pouch.

2. Transfer samples, for instance 10 µl of media from cells containing the secreted luciferase to opaque 96-microwell plates suitable for your luminometer.

3. Set up the luminometer prior to addition of 50 µl QUANTI-Luc™ reagent to each well either manually or by automated injection. Measure luminosity in endpoint mode when using Lucia luciferase or in kinetic mode depending on the coelenterazine-luciferase used.

Recent articles using InvivoGen QUANTI-Luc™文献

• 2017 - Sci Rep., 7(1):2683.
  RNAi Screen and Proteomics Reveal NXF1 as a Novel Regulator of IRF5 Signaling.
  Fu B. et al.
• 2016 - Nat Commun., 7:11492.
  Behavioural traits propagate across generations via segregated iterative-somatic and gametic epigenetic mechanisms.
  Mitchell E, Klein SL, Argyropoulos KV, Sharma A, Chan RB, Toth JG, Barboza L, Bavley C, Bortolozzi A, Chen Q, Liu B, Ingenito J, Mark W, Dudakov J, Gross S, Di Paolo G, Artigas F, van den Brink M, Toth M.
• 2016 - Methods Mol Biol., 1393:105-11.
  Exploiting secreted luciferases to monitor tumor progression in vivo.
  Falcone L, Casucci M.
• 2016 - J Neurosci., 36(5):1711-22.
  Principles Governing DNA Methylation during Neuronal Lineage and Subtype Specification.
  Sharma A, Klein SS, Barboza L, Lohdi N, Toth M.
• 2016 - Chem Commun (Camb)., [Epub ahead of print]
  Cellular delivery of enzyme-loaded DNA origami.
  Ora A. et al.

上海研卉生物科技有限公司 ，是一家专注于生命科学和生物技术领域的高科技企业，专业提供分子生物学、免疫学、生命科学基础研究以及临床检测等诸多领域的试剂、耗材、仪器等各类产品及生物技术服务。公司主营业务： 试剂盒：酶联免疫试剂盒、免疫印迹试剂盒、免疫组化试剂盒、细胞凋亡检测试剂盒、分子生物学试剂盒。 细胞：原装进口细胞、国产肿瘤细胞、国产正常细胞。 耗材：细胞培养耗材、普通实验耗材。 生化试剂：原装进口生化试剂、进口分装生化试剂、国产生化试剂。 抗体：免疫组化、免疫荧光、流式细胞检测、免疫细胞化学。