英文名称FoxP3
中文名称叉头蛋白P3抗体
别 名AIID; AIID; DIETER; DIETER; Forkhead box P3; Forkhead box protein P3; foxp3; foxp3; FOXP3_HUMAN; FOXP3delta7; Immune dysregulation polyendocrinopathy enteropathy X linked; Immunodeficiency polyendocrinopathy enteropathy X linked; IPEX; IPEX; JM2; JM2; MGC141961; MGC141963; OTTHUMP00000025832; OTTHUMP00000025833; OTTHUMP00000226737; PIDX; PIDX; SCURFIN; SCURFIN; XPID; XPID.
规格50ul 100ul 200ul
研究领域转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, Guinea Pig, 
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3ug/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量47kDa
细胞定位细胞核 
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human FoxP3:331-431/431 
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008].

Function:
Probable transcription factor. Plays a critical role in the control of immune response.

Subunit:
Interacts with IKZF3.

Subcellular Location:
Nucleus (Potential).

Post-translational modifications:
Acetylation on lysine residues stabilizes FOXP3 and promotes differentiation of T-cells into induced regulatory T-cells (iTregs) associated with suppressive functions. Deacetylated by SIRT1.

DISEASE:
Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.

Similarity:
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain.

SWISS:
Q9BZS1

Gene ID:
50943
Database links:
Entrez Gene: 50943 Human
Entrez Gene: 20371 Mouse
Entrez Gene: 317382 Rat
Omim: 300292 Human
SwissProt: Q9BZS1 Human
SwissProt: Q99JB6 Mouse
SwissProt: D3ZKI1 Rat
Unigene: 247700 Human
Unigene: 182291 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
叉头蛋白P3免疫组化荧光抗体
性 状：Lyophilized or Liquid
免 疫 原：KLH conjugated synthetic peptide derived from 
亚 型：IgG
纯化方法：affinity purified by Protein A
储 存 液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件：Store at -20 °C for one year.
叉头蛋白P3免疫组化荧光抗体，上海彩佑实业有限公司生产和代理的抗体和全国几十家高校、科研院所、制药公司、生物公司建立了长期的免疫技术服务，已成功完成数百个技术服务委托。
完善的服务流程，严格的质量管理体系，以确保每个项目高质量、高标准的按期完成。为科学家们提供最优质的、全方位的服务。
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服务目标:以国际高标准的标记服务水平，提供高品质的标记抗体产品，助力科研人员轻松科研。

叉头蛋白P3免疫组化荧光抗体，我公司可以按客户要求标记抗体，服务流程如下：
1. 客户提交委托标记请求并明确标记要求，与公司商定具体的实验项目、数量和费用，双方签订《委托标记制备合同》。
2. 客户提供待标记样品，我公司按照合同要求安排标记服务，并在规定时间内完成标记委托。
3. 标记完成后，我公司按照客户要求分装，免费低温邮寄给客户，同时提供标记报告。
样品要求：
1. 待标记样品需保证90%以上的纯度，样品中不含有干扰标记物结合的成分，如有特殊成分，请提前标注说明。
2. 待标记样品最小标记量为1mg，最好是干粉，如果是液体，浓度应大于1mg/ml。
3. 抗体IgG样品中应不含BSA、叠氮钠、甘氨酸等成分。
4. 大分子蛋白应提供分子量、等电点、缓冲液成分及pH等信息；小分子多肽需提供氨基酸序列；小分子化合物还需提供分子结构式。
5. 请提前说明任何特殊情况
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Sample: 
Spleen (Rat) Lysate at 40 ug
Primary: Anti-FoxP3 (bs-10211R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD


Protein: Raji(human) lysates at 40ug;
Primary: rabbit Anti-FoxP3 (bs-10211R) at 1:300; 
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; 
Predicted band size:47 kD
Observed band size:47 kD

Sample: 
Thymus (Mouse) Lysate at 40 ug
Primary: Anti-FoxP3 (bs-10211R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD


Sample: 
U937 Cell (Human) Lysate at 30 ug
Primary: Anti-FoxP3 (Bs-10211R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 48 kD


Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-FoxP3 Polyclonal Antibody, Unconjugated(bs-10211R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining


Tissue/cell: rat spleen cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-FoxP3 Polyclonal Antibody, Unconjugated(bs-10211R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining


Blank control (Black line):Mouse spleen (Black). 
Primary Antibody (green line): Rabbit Anti-FoxP3 antibody (bs-10211R) 
Dilution: 3μg /10^6 cells; 
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test. 
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA goat serum to block non-specific protein-protein interactions for 15 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.