General description

Detecting rare cells in circulatory system or bone marrow aspirates is difficult since such cells are diluted among many normal blood and bone marrow cells. However, the mRNA Isolation Kit for Blood/Bone Marrow and a companion reagent simplify the study of such rare cells (e.g., disseminated tumor cells for studying minimal residual disease) in several ways:

• The mRNA Isolation Kit for Blood/Bone Marrow increases the chance of finding transcripts from rare cells:
• It reproducibly yields large quantities of mRNA (variance in yield is less than 10%).
• It can concentrate the isolated mRNA up to 250-fold (from 5 mL of whole blood to a single 20 μL sample, which may be directly amplified in a single RT-PCR).
• It does notrequire an initial cell-separation step, thus minimizing the potential for loss of single or rare target cells.
• The mRNA Isolation Kit for Blood/Bone Marrow is intended for use with a companion reagent, the RNA/DNA Stabilization Reagent for Blood/Bone Marrow (sold separately), which stabilizes and lyses biological samples. This reagent immediately inactivates nucleases, as well as stabilizes cell lysates in the field for transport back to the laboratory. It also simplifies the study of rare cells by:
• Immediately lysing the cells, thereby "suspending" the metabolic state of target cells and the level of expressed mRNA in them. This shields the cellular nucleic acids from changes that might occur when cells are removed from their natural environment.
• Protecting mRNA (and DNA) from nucleases immediately after sample collection.

The mRNA Isolation Kit for Blood/Bone Marrow allows preparation of high-quality mRNA from stabilized blood or bone marrow samples.

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