• A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human IL-4 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
• Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
• One vial Biotin-labeled IL-4 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
• One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
• One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
• One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

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• A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
• An incubator that can provide stable incubation conditions up to 37°C±5°C.
• Squirt bottle, manifold dispenser, or automated microplate washer.
• Absorbent paper for blotting the microtiter plate.
• 100ml and 500ml graduated cylinders.
• Deionized or distilled water.

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