Recombinant Human ACE-2 (C419)

产品说明(Description)

Recombinant Human Angiotensin-Converting Enzyme 2 is produced by our Mammalian expression system and the target gene encoding Gln18-Ser740 is expressed with a 6His tag at the C-terminus. 

Accession #: Q9BYF1

Known as: Angiotensin-Converting Enzyme 2; ACE-Related Carboxypeptidase; Angiotensin-Converting Enzyme Homolog; ACEH; Metalloprotease MPROT15; ACE2

制剂（Formulation）

Supplied as a 0.2 μm filtered solution of 20mMTris，300mMNacl,1mMZncl2,10%Glycerol，PH7.4.

质量控制（Quality Control） 

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin:  Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

Bioactivity: 

Immobilized Human ACE-2-His(Cat#C419) at 10μg/ml (100 μl/well) can bind 2019-nCoV S Protein RBD-mFc (Cat#DRA32). The ED50 of Recombinant 2019-nCoV S Protein RBD-mFc (Cat#DRA32) is 62 ng/ml .

保存（Storage）

Store at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles.

背景（Background）

Angiotensin-Converting Enzyme 2 (ACE-2) is an integral membrane protein and a zinc metalloprotease of the ACE family, the ACE family includes somatic and germinal ACE. ACE-2 cleaves angiotensins I and II as a carboxypeptidase, ACE-2 converts angiotensin I to angiotensin 1-9, and angiotensin II to angiotensin 1-7. ACE-2 is also able to hydrolyze apelin-13 and *********-13 with high efficiency. ACE-2 can be high expressed in testis, kidney and heart, in colon, small intestine and ovary at moderate levels. Captopril and lisinopril as the classical ACE inhibitor don’t inhibit ACE-2 activity. ACE-2 may play an important role in regulating the heart function.