试剂盒性能: 1.样品线性回归与预期浓度相关系数R值为0.990以上。 2.批内与批见应分别小于9%和11% 检测范围: 50μIU/L -1000μIU/L 保存条件及有效期: 1.试剂盒保存:2-8℃ 2.有效期:6个月 FOR RESEARCH USE ONLY Drug Names Generic Name:Rat thyroid-stimulating hormone (TSH) ELISA Kit. Purpose This kit allows for the determination of TSH concentrations in Rat serum, blood plasma, and other biological fluids. Principle of the assay The kit assay Rat TSH level in the sample,use Purified Rat TSH to coat microtiter plate wells, make solid-phase antibody, then add TSH to wells, Combined TSH antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.