大鼠促甲状腺激素(TSH)酶联免疫分析(ELISA)试剂盒

¥1800
DAOYI
国内
2021-06-16 17:46

上海道壹生物科技有限公司

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上海道壹生物科技有限公司
王乐
18019269192 02124209195
shdaoyi@163.com
产品属性
数量大量
供应商道壹
检测限请索要产品说明书
检测方法请索要产品说明书
应用科研实验
标记物请索要产品说明书
样本血清、血浆、细胞上清液、尿液等
产品说明
大鼠促甲状腺激素(TSH)酶联免疫分析(ELISA)试剂盒
实验目的:本试剂盒仅供研究使用,用于测定大鼠血清,血浆及相关液体样本中促甲状腺激素(TSH)的含量。
实验原理:
本试剂盒应用双抗体夹心法测定标本中大鼠促甲状腺激素(TSH)水平。用纯化的大鼠促甲状腺激素(TSH)抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入促甲状腺激素(TSH),再与HRP标记的促甲状腺激素(TSH)抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的大鼠促甲状腺激素(TSH)呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),通过标准曲线计算样品中大鼠促甲状腺激素(TSH)浓度。 
试剂盒组成
试剂盒组成48孔配置96孔配置保存
说明书1份1份
封板膜2片(48)2片(96)
密封袋1个1个
酶标包被板1×481×962-8℃保存
标准品:1350μIU/L0.5ml×1瓶0.5ml×1瓶2-8℃保存
标准品稀释液1.5ml×1瓶1.5ml×1瓶2-8℃保存
酶标试剂3 ml×1瓶6 ml×1瓶2-8℃保存
样品稀释液3 ml×1瓶6 ml×1瓶2-8℃保存
显色剂A液3 ml×1瓶6 ml×1瓶2-8℃保存
显色剂B液3 ml×1瓶6 ml×1瓶2-8℃保存
终止液3ml×1瓶6ml×1瓶2-8℃保存
浓缩洗涤液(20ml×20倍)×1瓶(20ml×30倍)×1瓶2-8℃保存
试剂盒性能:
1.样品线性回归与预期浓度相关系数R值为0.990以上。
2.批内与批见应分别小于9%和11%
检测范围:
50μIU/L -1000μIU/L
保存条件及有效期:
1.试剂盒保存:2-8℃
2.有效期:6个月
FOR RESEARCH USE ONLY
Drug Names
Generic Name:Rat thyroid-stimulating hormone (TSH) ELISA Kit.
Purpose
This kit allows for the determination of TSH concentrations in Rat serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Rat TSH level in the sample,use Purified Rat TSH to coat microtiter plate wells, make solid-phase antibody, then add TSH to wells, Combined TSH antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.