恒河猴轮状病毒
ATCC  Number:VR-1739
Classification: Reoviridae, Rotavirus
Agent: Rhesus rotavirus (MMU 18006)
Strain: S:USA:79:2
Original Source: Stool sample from Macaca mulatta monkey with diarrhea. This product has been transferred into a different host at ATCC.
Depositors: RW Emmons and NJ Schmidt
Biosafety Level:2
Shipped: frozen
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Host Organism : Production Host: MA104.1 (ATCCCRL-2378.1) Alternate Hosts: BS-C-1 (ATCCCCL-26), Rhesus embryonic monkey kidney cells, Primary cynomolgus monkey kidney cells
Incubation : For best results, plate cells 24-48 hours in advance. Infect at 80%-90% confluent, not 100% confluent. Remove medium and inoculate with a small volume of virus diluted to provide a MOI of about 0.1. Adsorb 1 hour at 37.0°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubate at 37.0°C in a humidified, 5% CO2 atmosphere for 2-3 days, until CPE are well advanced in 100% of the culture. In TC, ATCCVR-1739 should be grown in serum free media that contains 2.0μg/ml trypsin.
Effect : CPE in the form of refractile, rounded cells throughout monolayer or in loose clusters.
Store at : -70.0°C or colder
Comments : Derived by adaptation of primary cell culture product ATCCVR-954 to MA104.1 (ATCCCRL-2378.1) cells. (Note: ATCCVR-954 and ATCCVR-1739 have not been compared with respect to sequence or infectivity in primary cell culture and tissue culture).
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