产品编号: KMP2416 产品名称: Mouse CD244 Protein, His Tag 中文名称: 重组鼠CD244蛋白 中文全称: 重组鼠自然杀伤细胞受体2B4蛋白 关键词: 自然杀伤细胞受体2B4, Natural killer cell receptor 2B4, NK cell activation-inducing ligand, NAIL, NKR2B4, h2B4, SLAM family member 4, SLAMF4, CD244, 2B4, Human CD244 Protein, His Tag, Recombinant Protein, KMP2416 物种: Mouse 宿主: HEK293 Cells 规格: 50ug, 100ug, 200ug 纯化方式: Affinity purification 纯度: >95% as determined by SDS-PAGE 内毒素水平: <1.0 EU/ug determined by the LAL method 缓冲体系: PBS, pH7.4 Uniprot: Q07763 SDS-PAGE: KMP2416 SDS-PAGE image1.png 功能: Heterophilic receptor of the signaling lymphocytic activation molecule (SLAM) family; its ligand is CD48. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Acts as activating natural killer (NK) cell receptor (PubMed:8326140, PubMed:12734329, PubMed:19648922, PubMed:20962259). Activating function implicates association with SH2D1A and FYN. Downstreaming signaling involves predominantly VAV1, and, to a lesser degree, SHIP1 and CBL. Signal attenuation in the absence of SH2D1A is proposed to be dependent on SHIP1 and to a lesser extent PTPN6/SHP-1 and PTPN11/SHP-2. Stimulates NK cell cytotoxicity, production of IFN-gamma and granule exocytosis (PubMed:8326140, PubMed:15169881, PubMed:15998796, PubMed:22683124). Optimal expansion and activation of NK cells seems to be dependent on the engagement of CD244 with CD48 expressed on neighboring NK cells (PubMed:15905190). Regulation of NK cell activity by adapters Sh2d1b and Sh2d1b2 is reported conflictingly (PubMed:16127454, PubMed:16425036). Acts as costimulator in NK activation by enhancing signals by other NK receptors such as NCR3 and NCR1. At early stages of NK cell differentiation may function as an inhibitory receptor possibly ensuring the self-tolerance of developing NK cells (By similarity). Involved in the regulation of CD8+ T-cell proliferation; expression on activated T-cells and binding to CD48 provides costimulatory-like function for neighboring T-cells (PubMed:11739483). Inhibits inflammatory responses in dendritic cells (DCs) (PubMed:25643613). 产品背景: Natural killer cell receptor 2B4(2B4/CD244)is a 66 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 protein family. SLAM family proteins have an extracellular domain(ECD) with two or four Ig-like domains and at least two cytoplasmic immunoreceptor tyrosine-based switch motifs(ITSMs). 2B4 interacts with CD48, while other SLAM family proteins interact in a homophilic manner. The mouse 2B4 cDNA encodes a 397 amino acid(aa) precursor that includes a 19 aa signal sequence, a 207 aa ECD with one Ig-like V-type and one C2-type Ig-like domain, a 21 aa transmembrane segment, and a 150 aa cytoplasmic domain with four ITSMs. Within the ECD, mouse 2B4 shares 46% and 68% aa sequence identity with human and rat 2B4, respectively. 2B4/CD48 signaling cooperates with other receptor systems to either promote or inhibit NK and CD8+ T cell activation. The inhibitory activities are distinct from those of MHC I restricted inhibitory NK cell receptors. Ligation of 2B4 with antibodies or CD48 constructs can directly trigger inhibitory signaling or disrupt an inhibitory interaction, leading to cellular activation. 2B4 can also induce signaling through CD48. 保存条件: Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. 运输条件: In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form. 说明: This product is for research use only. 官网链接: http://www.kmdbioscience.cn/ 参考文献: 1.J. Immunol. 167:6706-6710 (2001) 2.J. Immunol. 170:4881-4885 (2003) 3.Mol. Cell. Biol. 24:5144-5156 (2004) 4.J. Exp. Med. 202:181-192 (2005) 5.Nat. Immunol. 6:1002-1010 (2005) 6.Immunogenetics 58:15-25 (2006)