人S100钙结合蛋白钙粒蛋白A检测试剂盒 产品官方链接

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索莱宝
2026-04-27 09:38

北京索莱宝科技有限公司

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北京索莱宝科技有限公司
产品规格
规格编号 规格 库存 价格
SEKH-0421-96T 96T 现货 3200.00
SEKH-0421-48T 48T 现货 2000.00
产品说明
中文名称人S100钙结合蛋白钙粒蛋白A(S100A8)ELISA检测试剂盒
英文名称Human S100A8 ELISA Kit
别名S100 Calcium Binding Protein A8; S100A8; 60B8AG; CAGACP-10; Calgranulin A; Calgranulin-A; Calprotectin L1L Subunit; CFAG; CFAGL1Ag; CGLA; Cystic Fibrosis Antigen; MA387; MIF; MRP8; MRP-8; NIF; P8
储存条件保存在2-8℃,6个月。
规格96T ; 48T
运输条件冷藏运输
检测目标S100A8
样品类型血清/血浆/细胞培养上清
反应性Human
应用ELISA
反应类型Sandwich-ELISA
定量/定性定量检测
检测范围125-8000 pg/ml
灵敏度60 pg/ml
精密度板内,板间变异系数均<10%
回收率80%-120%
反应时间3h
Swiss ProtP05109
Gene ID6279
研究领域神经学
试剂盒类型传统ELISA试剂盒
检测原理Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人S100A8单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的人S100A8会与酶标板上的包被抗体充分结合;洗板后加入生物素化抗人S100A8抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的人S100A8发生特异性结合;洗板后加入辣根过氧化物酶(HRP)标记的链霉亲和素,生物素与链霉亲和素会发生高强度的非共价结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的人S100A8,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的人S100A8浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中人S100A8的浓度。
背景说明S100A8 (also MRP8 and calgranulin A) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca2+-dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Human S100A8 is 93 amino acids (aa) in length. It contains two EF-hand motifs (aa 12 - 47 and 46 - 81) and one high-affinity Ca2+-binding site (aa 59 - 70). There may be one splice form that shows a 15 aa substitution for the C-terminal 14 amino acids. Although mouse S100A8 is cleaved by MMP-2 after Asn21, it is unclear if human S100A8 is susceptible. Full-length human S100A8 is 57% and 74% aa identical to mouse and canine S100A8, respectively.
单位

Neutrophil-derived exosomal S100A8 aggravates lung injury in sepsis by inducing pyroptosis
Author:
Xinxin Li, Wei Zhou, Liangliang Zhou, Yingbin Li, Xufeng Wu, Jianjun Chen
IF:
3.2000
Publish_to:
MOLECULAR IMMUNOLOGY
PMID:
40056630