| 规格编号 | 规格 | 库存 | 价格 |
|---|---|---|---|
| SEKH-0421-96T | 96T | 现货 | 3200.00 |
| SEKH-0421-48T | 48T | 现货 | 2000.00 |
| 中文名称 | 人S100钙结合蛋白钙粒蛋白A(S100A8)ELISA检测试剂盒 |
| 英文名称 | Human S100A8 ELISA Kit |
| 别名 | S100 Calcium Binding Protein A8; S100A8; 60B8AG; CAGACP-10; Calgranulin A; Calgranulin-A; Calprotectin L1L Subunit; CFAG; CFAGL1Ag; CGLA; Cystic Fibrosis Antigen; MA387; MIF; MRP8; MRP-8; NIF; P8 |
| 储存条件 | 保存在2-8℃,6个月。 |
| 规格 | 96T ; 48T |
| 运输条件 | 冷藏运输 |
| 检测目标 | S100A8 |
| 样品类型 | 血清/血浆/细胞培养上清 |
| 反应性 | Human |
| 应用 | ELISA |
| 反应类型 | Sandwich-ELISA |
| 定量/定性 | 定量检测 |
| 检测范围 | 125-8000 pg/ml |
| 灵敏度 | 60 pg/ml |
| 精密度 | 板内,板间变异系数均<10% |
| 回收率 | 80%-120% |
| 反应时间 | 3h |
| Swiss Prot | P05109 |
| Gene ID | 6279 |
| 研究领域 | 神经学 |
| 试剂盒类型 | 传统ELISA试剂盒 |
| 检测原理 | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人S100A8单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的人S100A8会与酶标板上的包被抗体充分结合;洗板后加入生物素化抗人S100A8抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的人S100A8发生特异性结合;洗板后加入辣根过氧化物酶(HRP)标记的链霉亲和素,生物素与链霉亲和素会发生高强度的非共价结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的人S100A8,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的人S100A8浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中人S100A8的浓度。 |
| 背景说明 | S100A8 (also MRP8 and calgranulin A) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca2+-dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Human S100A8 is 93 amino acids (aa) in length. It contains two EF-hand motifs (aa 12 - 47 and 46 - 81) and one high-affinity Ca2+-binding site (aa 59 - 70). There may be one splice form that shows a 15 aa substitution for the C-terminal 14 amino acids. Although mouse S100A8 is cleaved by MMP-2 after Asn21, it is unclear if human S100A8 is susceptible. Full-length human S100A8 is 57% and 74% aa identical to mouse and canine S100A8, respectively. |
| 单位 | 盒 |