| 中文名称 | 大鼠基质金属蛋白酶3(MMP-3)ELISA检测试剂盒 |
| 英文名称 | Rat MMP-3 ELISA Kit |
| 别名 | CHDS6; matrix metallopeptidase 3 (stromelysin 1, progelatinase); MMP3; MMP-3; proteoglycanase; SL-1; STMY; STMY1MGC126104; STR1; Stromelysin 1; stromelysin-1; transin-1 |
| 储存条件 | 保存在2-8℃，6个月。 |
| 规格 | 96T ; 48T |
| 运输条件 | 冷藏运输 |
| 检测目标 | MMP-3 |
| 样品类型 | 血清/血浆/细胞培养上清 |
| 反应性 | Rat |
| 应用 | ELISA |
| 反应类型 | Sandwich-ELISA |
| 定量/定性 | 定量检测 |
| 检测范围 | 25-1600 pg/ml |
| 灵敏度 | 12.5 pg/ml |
| 特异性 | 不与大鼠BMP1,BMP2,BMP3,BMP4,IL-2,IL-4,IL-5,IL-6,IFN-γ,TGF-β1,TGF-β2,TGF-β3,TLR1,TLR2,TLR3,TNF-α等反应。 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 反应时间 | 3.5h |
| Swiss Prot | P03957 |
| Gene ID | 171045 |
| 疾病系列 | 骨关节炎相关因子 |
| 研究领域 | 信号传导 ; 细胞生物学 ; 心血管 |
| 试剂盒类型 | 传统ELISA试剂盒 |
| 检测原理 | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠MMP-3单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的大鼠MMP-3会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗大鼠MMP-3抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠MMP-3发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠MMP-3，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠MMP-3浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠MMP-3的浓度。 |
| 背景说明 | The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).
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| 单位 | 盒 |
