大鼠基质金属蛋白酶14检测试剂盒 产品官方链接

2600.00
索莱宝
2026-05-07 10:28

北京索莱宝科技有限公司

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龚思雨
18101056239 010-50973130
3193328036@qq.com
3193328036
北京索莱宝科技有限公司
产品规格
规格编号 规格 库存 价格
SEKR-0070-96T 96T 现货 2600.00
SEKR-0070-48T 48T 现货 1600.00
产品说明
中文名称大鼠基质金属蛋白酶14(MMP-14)ELISA检测试剂盒
英文名称Rat MMP-14 ELISA Kit
别名matrix metallopeptidase 14 (membrane-inserted); matrix metalloproteinase-14; membrane type 1 metalloprotease Membrane-type matrix metalloproteinase 1; MP14; MMP-14; MMP-X1; MT1MMP; MT1-MMP; MT-MMP 1
储存条件保存在2-8℃,6个月。
规格96T ; 48T
运输条件冷藏运输
检测目标MMP-14
样品类型血清/血浆/细胞培养上清
反应性Rat
应用ELISA
反应类型Sandwich-ELISA
定量/定性定量检测
检测范围250-16000 pg/ml
灵敏度125 pg/ml
特异性不与大鼠ApoAI,BMP1,BMP2,BMP3,BMP4,BMP7,CRP,HGF,HSP27,IL-1α,IL-1RI,IL-1β,IL1RA,IL-2,IL-4,IL-5,IL-6,IL-10,IL-12,IL-13,IL-15,IL-17C,IL-21,IL-23,IFN ,MMP-2,sIL-2R,sIL-6R,PDGF,PLA2G7,prolactin,TGFβ1,TGFβ2,TGFβ3,TLR1,TLR2,TLR3等反应。
精密度板内,板间变异系数均<10%
回收率80%-120%
反应时间3.5h
Swiss ProtQ10739
Gene ID81707
试剂盒类型传统ELISA试剂盒
检测原理Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠MMP-14单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的大鼠MMP-14会与酶标板上的包被抗体充分结合;洗板后加入生物素化抗大鼠MMP-14抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠MMP-14发生特异性结合;洗板后加入辣根过氧化物酶(HRP)标记的链霉亲和素,生物素与链霉亲和素会发生高强度的非共价结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的大鼠MMP-14,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的大鼠MMP-14浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠MMP-14的浓度。
背景说明As the first member of membrane type (MT) MMPs, MMP-14, also known as MT1-MMP, plays an important role in extracellular matrix (ECM) remodeling by being able to degrade type I collagen, activate pro-MMP-2 and process cell adhesion molecules such as CD44 and integrin alpha V. MMP-14 is therefore a key enzyme in many physiological and pathological processes such as angiogenesis and tumor invasion. Structurally, MMP-14 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplamasic tail. Recombinant Human MMP-14 consists of the pro and catalytic domains, which can be activated by treatment with furin as described in the Activity Assay Protocol.
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