小鼠甲胎蛋白检测试剂盒 产品官方链接

2000.00
索莱宝
2025-12-10 01:26

北京索莱宝科技有限公司

VIP会员
龚思雨
18101056239 010-50973130
3193328036@qq.com
3193328036
北京索莱宝科技有限公司
产品规格
规格编号 规格 库存 价格
SEKM-0174-48T 48T 现货 2000.00
SEKM-0174-96T 96T 现货 3200.00
产品说明
中文名称小鼠甲胎蛋白(AFP)ELISA检测试剂盒
英文名称Mouse AFP ELISA Kit
别名AFP; alpha-1-fetoprotein; Alpha-fetoglobulin; alpha-Fetoprotein; DSCAM2; FETA; HP; HPAFP
储存条件保存在2-8℃,6个月。
规格48T ; 96T
运输条件冷藏运输
检测目标AFP
样品类型血清/血浆/细胞培养上清
反应性Mouse
应用ELISA
反应类型Sandwich-ELISA
定量/定性定量检测
检测范围62.5-4000 pg/ml
灵敏度31.25 pg/ml
特异性不与小鼠Leptin,Prolactin,Resistin,VitaminDBP,人AFP等反应。
精密度板内,板间变异系数均<10%
回收率80%-120%
反应时间3h
Swiss ProtP02772
Gene ID11576
研究领域细胞生物学 ; 心血管 ; 神经学 ; 肿瘤&癌症
试剂盒类型传统ELISA试剂盒
检测原理Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗小鼠AFP单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的小鼠AFP会与酶标板上的包被抗体充分结合;洗板后加入生物素化抗小鼠AFP抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的小鼠AFP发生特异性结合;洗板后加入辣根过氧化物酶(HRP)标记的链霉亲和素,生物素与链霉亲和素会发生高强度的非共价结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的小鼠AFP,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的小鼠AFP浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中小鼠AFP的浓度。
背景说明alpha-Fetoprotein (AFP), is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver, yolk sac, and tissues of gastrointestinal origin. It is one of the earliest markers of the hepatocyte lineage. AFP acts as a carrier protein for steroids, bilirubin, fatty acids, retinoids, and flavonoids. In addition, it can exert immunosuppressive activity, regulate cell proliferation and apoptosis, initiate intracellular signaling, and contribute to cell invasion. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. Low maternal serum AFP levels are associated with a higher incidence of Down syndrome, whereas higher levels are associated with spina bifida and anencephaly. Elevated AFP levels are also coincident with liver, stomach, and germ cell cancers.
单位

Exosomes derived from Baicalin-pretreated bone mesenchymal stem cells improve Th17/Treg imbalance after hepatic ischemia–reperfusion via FGF21 and the JAK2/STAT3 pathway
Author:
Baoyan Zhang, Linfeng Su, Zhichao Chen, Min Wu, Jianfeng Wei, Yonghua Lin
IF:
4.6000
Publish_to:
IUBMB LIFE
PMID:
38380586