| 别名 | Lysine--tRNA Ligase;Lysyl-tRNA Synthetase;LysRS;KARS;KIAA0070 |
| 英文名称 | Recombinant Human Lysine--tRNA ligase/KARS |
| 中文名称 | 重组人Lysine--tRNA ligase/KARS |
| 规格 | 10ug ; 50ug ; 500ug ; 1mg |
| 外观(性状) | Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 100mM NaCl, 1mM DTT, 20% Glycerol, pH 8.0. |
| 纯度 | ≥95% as determined by reducing SDS-PAGE. |
| 储存条件 | Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 运输条件 | 干冰运输 |
| 背景说明 | Lysine-tRNA ligase，also known as Lysyl-tRNA synthetase，LysRS，KARS and KIAA0070，belongs to the class-II aminoacyl-tRNA synthetase family.The N-terminal cytoplasmic domain (1-65) is a functional tRNA-binding domain，which is required for nuclear localization，is involved in the interaction with DARS，but has a repulsive role in the binding to EEF1A1.A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions.KARS catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.Defects in KARS are the cause of Charcot-Marie-Tooth disease recessive intermediate type B (CMTRIB). |
| 来源 | Human Cells |
| 内毒素 | Less than 0.1 ng/ug(1 EU/ug)as determined by LAL test. |
| 单位 | 支 |
