GPCR GPR120 Antibody (Ab106082) - Western Blot
All lanes: GPCR GPR120 Antibody (Ab106082) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 40 kDa
Observed band size: 57, 43 kDa
Exposure time: 75.5 s

GPCR GPR120 Antibody (Ab106082) - IHC
Immunohistochemical analysis of GPR120 staining in human prostate formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with GPCR GPR120 Antibody (Ab106082) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

GPCR GPR120 Antibody (Ab106082) - IF/ICC
Immunofluorescent analysis of GPR120 staining in LO2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with GPCR GPR120 Antibody (Ab106082) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin-AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).