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| 规格编号 | 规格 | 库存 | 价格 |
|---|---|---|---|
| Ab133739-100μl | 100μl | 期货 | 1819.90 |
| Ab133739-10μl | 10μl | 现货 | 399.90 |
| Ab133739-1ml | 1ml | 期货 | 13999.90 |
| Ab133739-50μl | 50μl | 现货 | 1199.90 |
规格或纯度：ExactAb™, Validated, Carrier Free, 1.0 mg/mL
特异性：USP30
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat), 狗(Dog), 猴(Monkey)
免疫原：A synthetic peptide derived from human USP30
阳性对照：WB: Mouse liver, Rat liver, Hep G2, MCF7, A549 and NIH/3T3 lysates. ICC/IF: HeLa cells.
偶联：Unconjugated
克隆类型：多克隆抗体
Format：Whole IgG
亚型：Rabbit IgG
SDS-PAGE：150 kDa
纯化方法：Immunogen affinity purified
物理外观：Liquid
储存缓冲液：0.42% Potassium phosphate, 0.87% Sodium chloride, 30% Glycerol, 0.01% Sodium azide, pH 7.3
防腐剂： 0.01% Sodium azide
浓度：1.0 mg/mL
储存温度：-20°C储存,避免反复冻融
运输条件：超低温冰袋运输
稳定性与储存：4°C 短期储存（1-2 周）。-20°C下长期保存（12个月）。交付时等分。避免冷冻/解冻循环。
Recombinant USP30 Antibody (Ab133739) - Western Blot
All lanes: Recombinant USP30 Antibody (Ab133739) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/40000 dilution
Predicted band size: 59 kDa
Observed band size: 61 kDa
Exposure time: 3.9 s
Recombinant USP30 Antibody (Ab133739) - IF
Immunofluorescent analysis of USP30 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the Recombinant USP30 Antibody (Ab133739) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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