Active Rheb GTP  

[30 µL] 

Catalog Number:  CR-M26910 

Gene Symbol:  RHEB 

Description:   Anti Active Rheb Mouse Monoclonal Antibody

Background:   Small GTPases are a super-family of cellular signaling regulators. Rheb is a member of the Ras-superfamily GTPases. Rheb has been shown to interact with C-Raf, Mammalian target of rapamycin (mTOR), TSC2, Ataxia telangiectasia mutated (ATM), KIAA1303 and Ataxia telangiectasia and Rad3 related (ATR). Immunogen:   Recombinant full length protein of active Rheb. Applications:   IP, IHC Recommended Dilutions:   1 μg for 1~2 mg total cellular proteins for IP 1:100 dilution for IHC Concentration:   1 mg/ml Host:   mouse Format:   Liquid Clonality:   Monoclonal Isotype:   IgM Purity:   Purified from ascites Storage buffer: Preservative:  no Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity:  Anti active Rheb antibody recognizes active Rheb of vertebrates. Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles 

Immunoprecipitation/Western blot:

Rheb activation assay. Purified Rheb proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti active Rheb monoclonal antibody (Cat. No. CR-M26910). Immunoblot was with an anti Rheb rabbit polyclonal antibody (Cat. No.CR-M 21098).