Active Rap1-GTP  

[30 µL] 

Catalog Number:  CR-M26912 

Gene Symbol:  RAP1 

Description:   Anti Active Rap1 Mouse Monoclonal Antibody 

Background:   Small GTPases are a super-family of cellular signaling regulators. Rap family members (Rap1A, Rap1B, Rap2A, Rap2B, and Rap2C) are Ras-like GTPases that regulate cell proliferation, differentiation, apoptosis and adhesion mechanisms. Rap1 (Ras-proximate-1) regulates apoptosis through interaction with two MAP3K effectors of the MEK/ERK MAP-kinase pathway, Raf-1 and B-Raf. Rap1 regulates cell adhesion mechanisms through effector molecules such as the integrins and cadherins. Immunogen:   Recombinant full length protein of active Rap1. Applications:   IP, IHC Recommended Dilutions:   1 μg for 1~2 mg total cellular proteins for IP 1:100 dilution for IHC Concentration:   1 mg/ml Host:   mouse Format:   Liquid Clonality:   Monoclonal Isotype:   IgG Purity:   Purified from ascites Storage buffer: Preservative:  no Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity:  Anti-active Rap1 antibody recognizes active Rap1 of vertebrates. Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles Immunoprecipitation/Western blot:

Rap1 activation assay. Purified Rap1 proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-active Rap1 monoclonal antibody (Cat. No. CR-M26912). Immunoblot was with an anti-Rap1 polyclonal antibody (Cat. No. CR-M21104).