Active Ral GTP  

[30 µL] 

Catalog Number:  CR-M26913 

Gene Symbol:  Ral 

Description:   Anti Active Ral Mouse Monoclonal Antibody

Background:   The Ras-like small G proteins, RalA and RalB, are important components of Ras signaling pathways, implicated in the initiation and maintenance of tumorigenic transformation, as well as vesicle transport, apoptosis, transcription, cell migration, and cell proliferation. Immunogen:   Recombinant full length protein of active RalB. Applications:   IP, IHC Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP;  1:100 dilution for IHC Concentration:   1 mg/ml Host:   mouse Format:   Liquid Clonality:   Monoclonal Isotype:   IgG Purity:   Purified from ascites Storage buffer: Preservative:  no Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity:  Anti-active Ral antibody recognizes active RalA and RalB of vertebrates. Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles Download Datasheet:   

Immunoprecipitation/Western blot:

Ral activation assay. Purified RalB proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti active Ral monoclonal antibody (Cat. No. CR-M26913). Immunoblot was with an anti RalB rabbit polyclonal antibody (Cat. No. CR-M21033).